Vitis has two subgenera: Euvitis, which includes commercially important Vitis vinifera and interspecific hybrid cultivars, and Muscadinia. Of note, the market for Muscadinia grapes remains small, and only Muscadinia rotundifolia is cultivated as a commercial crop. To establish a basis for the study of Muscadinia species, we generated chromosome-level whole-genome sequences of Muscadinia rotundifolia cv. Noble. A total of 393.8 Mb of sequences were assembled from 20 haploid chromosomes, and 26 394 coding genes were identified from the sequences. Comparative analysis with the genome sequence of V. vinifera revealed a smaller size of the M. rotundifolia genome but highly conserved gene synteny. A genome-wide association study of 12 Muscadinia berry-related traits was performed among 356 individuals from breeding populations of M. rotundifolia. For the transferability of markers between Euvitis and Muscadinia, we used 2000 core genome rhAmpSeq markers developed to allow marker transferability across Euvitis species. A total of 1599 (80%) rhAmpSeq markers returned data in Muscadinia. From the GWAS analyses, we identified a total of 52 quantitative trait nucleotides (QTNs) associated with the 12 berry-related traits. The transferable markers enabled the direct comparison of the QTNs with previously reported results. The whole-genome sequences along with the GWAS results provide a new basis for the extensive study of Muscadinia species.

中文翻译：

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Muscadinia rotundifolia 的染色体水平基因组序列组装和全基因组关联研究揭示了 12 个浆果相关性状的遗传学。


葡萄有两个亚属：Euvitis，其中包括商业上重要的葡萄和种间杂交品种，以及Muscadinia。值得注意的是，麝香葡萄的市场仍然很小，只有圆叶麝香被作为经济作物种植。为了为麝香菌物种的研究奠定基础，我们生成了圆叶麝香菌的染色体水平全基因组序列。高贵。由20条单倍体染色体组装出总共393.8 Mb的序列，并从序列中鉴定出26 394个编码基因。与V. vinifera 基因组序列的比较分析表明，M. rotundifolia 基因组较小，但基因同线性高度保守。对来自圆叶麝香繁殖种群的 356 个个体进行了 12 个麝香浆果相关性状的全基因组关联研究。对于Euvitis和Muscadinia之间标记的可转移性，我们使用了2000个核心基因组rhAmpSeq标记，该标记是为了允许标记在Euvitis物种之间转移而开发的。总共 1599 个 (80%) rhAmpSeq 标记返回了 Muscadinia 的数据。通过 GWAS 分析，我们总共鉴定了 52 个与 12 个浆果相关性状相关的数量性状核苷酸 (QTN)。可转移标记能够将 QTN 与之前报告的结果进行直接比较。全基因组序列以及 GWAS 结果为蝇类物种的广泛研究提供了新的基础。