Abstract

MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced MYB transcription factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 was localized to the nucleus and exhibited transactivational activity. Overexpression of DgMYB2 improved cold tolerance in chrysanthemum, while cold tolerance in the antisense suppression lines decreased compared to that of the wild type. Additionally, electrophoretic mobility shift assays, chromatin immunoprecipitation, luciferase complementary imaging analysis, and dual-luciferase reporter gene detection experiments confirmed that DgMYB2 directly targets DgGPX1 and increases the activity of glutathione peroxidase to reduce the accumulation of reactive oxygen species, thereby improving cold resistance in chrysanthemum.

中文翻译：

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DgMYB2直接靶向DgGPX1提高菊花的抗寒性

 抽象的

MYB转录因子在植物对冷胁迫的反应中发挥重要作用，但相关的潜在机制仍不清楚。在这项研究中，从菊花（ Chrysanthemum morifolium Ramat）中分离出一种冷诱导的 MYB 转录因子DgMYB2 。 DgMYB2 定位于细胞核并表现出反式激活活性。 DgMYB2的过表达提高了菊花的耐冷性，而反义抑制系的耐冷性与野生型相比下降。此外，电泳迁移率变动分析、染色质免疫沉淀、荧光素酶互补成像分析和双荧光素酶报告基因检测实验证实，DgMYB2直接靶向DgGPX1 ，并增加谷胱甘肽过氧化物酶的活性，减少活性氧的积累，从而提高抗寒能力。菊花。