In the present study, xylooligosaccharides (XOS) production was carried out using sorghum (Sorghum bicolor (L.)) xylan and xylanase from isolated Aspergillus fumigates RSP-8 followed by evaluation of their prebiotic potential using Lactobacillus sp. T-10. Isolated xylan revealed structural similarity to Birchwood xylan and is composed of xylose (89%) arabinose (6%) and glucose (5%). Xylanase enzyme produced by Aspergillus fumigatus RSP-8 revealed 43 kDa molecular weight with little β-glucosidase and depicted optimum activity of 1248 U/mL at pH 5.0 and temp 50 °C. Time-dependent XOS [(X2 (xylobiose) to X7 (xyloheptose)] production was noticed and differed from source to source. XOS production kinetics indicated that maximum XOS (8.4 g reducing sugar/L) was obtained in 12 h at 50 °C with 50 U/mL xylanase with 1.5% (w/v) sorghum xylan as substrate. The produced XOS promoted Lactobacillus T-10 growth indicating its prebiotic potential. This study can provide useful insights into the processes required for further upscaling of agricultural biomass to nutraceutical product production and the industrial application potential of sorghum xylan.

在本研究中，使用来自分离的烟曲霉RSP-8 的高粱 ( Sorghum bicolor (L.)) 木聚糖和木聚糖酶进行低聚木糖 (XOS) 的生产，然后使用乳酸杆菌 ( Lactobacillus sp.) 评估其益生元潜力。T-10。分离的木聚糖揭示了与桦木木聚糖的结构相似性，由木糖 (89%) 阿拉伯糖 (6%) 和葡萄糖 (5%) 组成。烟曲霉产生的木聚糖酶RSP-8 的分子量为 43 kDa，几乎没有 β-葡萄糖苷酶，在 pH 5.0 和温度 50 °C 下的最佳活性为 1248 U/mL。随时间变化的 XOS [(X2 (木二糖) 到 X7 (木庚糖)] 的生产受到关注，并且因来源而异。XOS 生产动力学表明，在 50 °C 下 12 小时内获得最大 XOS (8.4 g 还原糖/L)以 50 U/mL 木聚糖酶和 1.5% (w/v) 高粱木聚糖为底物。产生的 XOS 促进了乳酸杆菌T-10 的生长，表明其益生元潜力。这项研究可以为进一步扩大农业生物质规模所需的过程提供有用的见解高粱木聚糖的保健品生产和工业应用潜力。