Biomass Conversion and Biorefinery ( IF 3.5 ) Pub Date : 2022-01-17 , DOI: 10.1007/s13399-022-02301-x Shunmugiah Mahendran 1 , Senthurpandian Muthuramalinga Sethupathi 1 , Subbiah Sankaralingam 2 , Durairaj Kathiresan 2 , Mahalingam Muthumani 2 , Loganathan Kousalya 3 , Selvam Palpperumal 4 , Balasundaram Harinathan 4
The polyphenol compound is extracted from Sargassum tenerrimum with various bioactivities including antibacterial and antioxidant activity and MTT assay for cell cytotoxicity. The total phenolic content was 69.12 ± 0.24%. The S. tenerrimum polyphenol was found to phytochemical constituent’s presence of flavonoids, saponins, tannins, phenolics, alkaloids and steroid. The antibacterial activity of polyphenol presented significant inhibition against ten human pathogen bacterial cultures such as Proteus mirabilis, Klebsiella oxytoca, Escherichia coli, Bacillus cereus, Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella typhi and Bacillus subtilis. The in vitro antioxidant activity and MTT assay revealed that the polyphenol has anticancer activity against HeLa cells. The polyphenol compound was characterized through HPLC.
中文翻译:
褐海藻马尾藻生物质多酚的抗氧化和细胞毒活性评价
多酚化合物是从马尾藻中提取的,具有多种生物活性,包括抗菌和抗氧化活性以及用于细胞毒性的 MTT 测定。总酚含量为 69.12 ± 0.24%。S.tenerrimum多酚被发现植物化学成分的黄酮类化合物、皂苷、单宁、酚类、生物碱和甾体的存在。多酚的抗菌活性对奇异变形杆菌、催产克雷伯菌、大肠杆菌、蜡状芽孢杆菌、化脓性链球菌、金黄色葡萄球菌、铜绿假单胞菌、霍乱弧菌、伤寒沙门氏菌和枯草芽孢杆菌。体外抗氧化活性和MTT测定表明多酚对HeLa细胞具有抗癌活性。通过HPLC表征多酚化合物。