Background

The demand for Coenzyme Q10 (CoQ10) to the human body is increasing due to its potential health function. However, the production of CoQ10 in yields remains a big problem for commercialization.

Methods

This study attempts to develop a useful fermentation strategy for enhancing the production of CoQ10 from the Rhodobacter sphaeroides.

Significant Findings

It was observed that the cell growth, the concentration and the content of CoQ10 were further enhanced to 11.44 g/L, 60.02 mg/L and 5.25 mg/g by the assimilation of 2 g/L of vitamin B1. This fed-batch culture using vitamin B1 as a feeding substrate resulted into the cell growth, the CoQ10 production and the CoQ10 content of 16.5 g/L, 88.7 mg/L and 5.38 mg/g respectively. Moreover, the fed-batch culture with 100% medium replacement of 12 h-, 24 h- and 48 h-cultivations were also performed. The maximal cell growth, CoQ10 production and CoQ10 content were found to be 37.65 g/L, 138.24 mg/L and 3.67 mg/g, respectively. Eventually, this study also identified the CoQ10 produced by R. sphaeroides using FAB-MS and ¹HNMR . It was suggested that by optimizing the environmental factors, the CoQ10 production was enhanced by 3.42 folds for fed-batch culture when compared with the batch cultivation.

中文翻译：

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使用潜在的发酵策略增强球形红杆菌辅酶 Q10 的生产和表征

背景

由于辅酶Q10（CoQ10）具有潜在的保健功能，人体对辅酶Q10（CoQ10）的需求正在增加。然而，CoQ10 的产量仍然是商业化的一个大问题。

方法

本研究试图开发一种有用的发酵策略，以提高球形红杆菌的 CoQ10 产量。

重要发现

观察到通过同化2 g/L的维生素B1，细胞生长、浓度和CoQ10含量进一步提高至11.44 g/L、60.02 mg/L和5.25 mg/g。这种以维生素 B1 为补料底物的分批补料培养导致细胞生长、CoQ10 产量和 CoQ10 含量分别为 16.5 g/L、88.7 mg/L 和 5.38 mg/g。此外，还进行了 12 小时、24 小时和 48 小时培养的 100% 培养基更换的补料分批培养。最大细胞生长、辅酶Q10产量和辅酶Q10含量分别为37.65 g/L、138.24 mg/L和3.67 mg/g。最终，本研究还使用 FAB-MS 和^{1鉴定了球状}红球菌产生的 CoQ10核磁共振。建议通过优化环境因素，与分批培养相比，补料分批培养的辅酶Q10产量提高了3.42倍。