Background

Ionic liquids (IL) is a unique environmental benign solvent with attractive characteristics which can be used in the formation of aqueous biphasic system. Aqueous biphasic flotation (ABF) system can improve the recovery yields of target biomolecules by incorporating the gas bubbles into the aqueous biphasic system (ABS). Keratinase has been widely applied in numerous industries due to the capability to degrade the keratinous wastes and convert them into valuable products via bioconversion.

Methods

The feasibility of IL-based ABF system to recover the Kytococcus sedentarius TWHKC01 keratinase from crude feedstock was investigated in this study.

Findings

IL-based ABF system formed with 25.0% (w/w) 1-ethyl-3-methylimidazolium ethyl sulphate, (EMIM)(ESO₄), 12.5% (w/w) sodium carbonate and 20% (w/w) crude feedstock demonstrated the optimum yield (Y) of 91.28% ± 0.64 and partition coefficient (K_e) of 8.39 ± 0.64. Keratinase was transferred to the IL-rich top phase of the ABF system which was operating at an airflow rate of 20 cc/min for 5 min. The keratinase selectivity (S) of 2.48 ± 0.31 and purification fold (PF) of 2.12 ± 0.04 were recorded. The IL-based ABF system is therefore highly recommended as a one-step operation to recover and purify the microbial keratinase due to the cost-saving and environmentally friendly properties.

中文翻译：

---

离子液体/盐水溶液双相浮选系统从粗原料中回收久坐猕猴桃 TWHKC01 角蛋白酶的评价

背景

离子液体 (IL) 是一种独特的环境良性溶剂，具有吸引人的特性，可用于水性双相体系的形成。水相双相浮选 (ABF) 系统可以通过将气泡结合到水相双相系统 (ABS) 中来提高目标生物分子的回收率。角蛋白酶由于能够降解角蛋白废物并通过生物转化将其转化为有价值的产品，因此已广泛应用于许多行业。

方法

本研究研究了基于 IL 的 ABF 系统从粗原料中回收Kytococcus sedentarius TWHKC01 角蛋白酶的可行性。

发现

由 25.0% (w/w) 1-乙基-3-甲基咪唑鎓乙基硫酸盐、(EMIM)(ESO ₄ )、12.5% (w/w) 碳酸钠和 20% (w/w) 粗品形成的基于 IL 的 ABF 系统原料的最佳收率 (Y) 为 91.28% ± 0.64，分配系数 (K _e ) 为 8.39 ± 0.64。将角蛋白酶转移到 ABF 系统的富含 IL 的顶部相中，该系统以 20 cc/min 的气流速率运行 5 分钟。记录了 2.48 ± 0.31 的角蛋白酶选择性 (S) 和 2.12 ± 0.04 的纯化倍数 (PF)。因此，强烈推荐基于 IL 的 ABF 系统作为回收和纯化微生物角蛋白酶的一步操作，因为它具有成本节约和环境友好的特性。