This study aimed to evaluate the effect of supplementing arginine (Arg) + glutamine (Gln) replacing antibiotics on performance, immune response, and antioxidant capacity of pigs in the growing phase. One hundred fifty 63-d-old pigs with initial body weight (BW) of 25.0 ± 1.46 kg were distributed in a randomized block design, with three treatments and ten replicates. The three diets were control; antibiotic, control + 100 mg/kg tiamulin and 506 mg/kg oxytetracycline; amino acid, control + 10 g/kg Arg and 2 g/kg Gln. Dietary treatments were fed from 63 to 77 d. Following the treatment period, all pigs were fed the control diet from 77 to 90 d. Data were analyzed using GLIMMIX and UNIVARIATE in SAS 9.4. From 63 to 70 d, pigs fed diets with antibiotics had improved (P < 0.05) average daily feed intake, average daily weight gain (ADG), gain to feed ratio (G:F), and 70-d BW compared to those fed control or amino acid diets. From 70 to 77 d, including antibiotics in the diet increased (P < 0.05) ADG and 77-d BW. From 77 to 90 d, pigs fed control or amino acid diets had greater (P < 0.05) ADG than those fed an antibiotic diet. From 63 to 90 d, although pig performance was not affected (P > 0.05), growth curve of pigs fed the antibiotic diets was different (P < 0.05) from those fed the control and amino acids diets. At 70 d, serum tumor necrosis factor-α and diamine oxidase (DAO) were lower (P < 0.05) in pigs fed the antibiotic diet than the control diet, and pigs fed the amino acid diet had intermediate results. Ferric reducing antioxidant power (FRAP) was lower (P < 0.05) in pigs fed the amino acid diet than the antibiotic diet, and pigs fed the control diet had intermediate results. Serum immunoglobulin A was lower (P < 0.05) in pigs fed the antibiotic diet. At 77 d, DAO and serum immunoglobulin G were lower (P < 0.05) in pigs fed the antibiotic diet. FRAP was lower (P < 0.05) in pigs fed the amino acid and control diets. Serum malondialdehyde was higher (P < 0.05) in pigs fed the amino acid diet than those fed the control diet, and pigs fed the antibiotic diet had intermediate results. At 90 d, antibiotics or amino acids did not affect (P > 0.05) serum parameters. Amino acid blend supplementation at the selected doses in this study did not positively affect growing pigs. Although from 63 to 77 d, antibiotics improved performance, when considering the overall study period, growing pigs did not benefit from a diet containing antibiotics.

中文翻译：

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氨基酸混合物替代抗生素对生长猪的影响

本研究旨在评估补充精氨酸 (Arg) + 谷氨酰胺 (Gln) 替代抗生素对生长期猪的生产性能、免疫反应和抗氧化能力的影响。将 150 头初始体重 (BW) 为 25.0 ± 1.46 kg 的 63 日龄猪分配到随机区组设计中，分为 3 个处理和 10 个重复。控制三种饮食；抗生素，对照 + 100 mg/kg 泰妙菌素和 506 mg/kg 土霉素；氨基酸，对照 + 10 g/kg Arg 和 2 g/kg Gln。从 63 到 77 天喂食日粮。治疗期后，所有猪在 77 至 90 天喂食对照日粮。在 SAS 9.4 中使用 GLIMMIX 和 UNIVARIATE 分析数据。从 63 到 70 天，饲喂含抗生素日粮的猪的平均日采食量、平均日增重（ADG）均得到改善（P < 0.05），与饲喂对照或氨基酸饮食的那些相比，增重比 (G:F) 和 70 天体重。从 70 到 77 天，包括饮食中的抗生素增加 (P < 0.05) 平均增重和 77 天体重。从 77 天到 90 天，饲喂对照日粮或氨基酸日粮的猪比饲喂抗生素日粮的猪具有更高 (P < 0.05) ADG。从 63 到 90 天，虽然猪的生产性能没有受到影响（P > 0.05），但饲喂抗生素日粮的猪的生长曲线与饲喂对照日粮和氨基酸日粮的猪的生长曲线不同（P < 0.05）。70天时，饲喂抗生素日粮的猪血清肿瘤坏死因子-α和二胺氧化酶（DAO）低于对照日粮（P < 0.05），饲喂氨基酸日粮的猪结果居中。饲喂氨基酸日粮的猪的铁还原抗氧化能力 (FRAP) 低于抗生素日粮 (P < 0.05)，喂食对照饮食的猪有中间结果。饲喂抗生素饮食的猪的血清免疫球蛋白 A 较低 (P < 0.05)。在 77 天时，饲喂抗生素日粮的猪的 DAO 和血清免疫球蛋白 G 较低 (P < 0.05)。饲喂氨基酸日粮和对照日粮的猪的 FRAP 较低 (P < 0.05)。饲喂氨基酸日粮的猪的血清丙二醛含量高于饲喂对照日粮的猪 (P < 0.05)，而饲喂抗生素日粮的猪的结果居中。在 90 天时，抗生素或氨基酸不影响 (P > 0.05) 血清参数。在本研究中以选定剂量补充氨基酸混合物并未对生长猪产​​生积极影响。尽管从 63 天到 77 天，抗生素提高了生产性能，但考虑到整个研究期间，生长猪并没有从含有抗生素的饮食中获益。