Effective utilization of waste paper as a substrate for biodiesel production has environmental and economic advantages. To study the feasibility of using waste office automation paper to produce microbial lipids, waste paper hydrolysate (WPH) was prepared by enzymatic hydrolysis. WPH was fed to Rhodococcus opacus PD630 for microbial lipid production. Among the nitrogen sources tested, the medium with NH₄Cl yielded significantly high biomass and lipid of 4.92 and 1.29 g/L, respectively. When the ratio of C/N increases from 10 to 80, the lipid accumulation increases and the lipid content also increases from 18.92 to 43.41%. Under optimized conditions, the maximum biomass, lipid yield, and lipid content obtained were 6.51 g/L, 2.8 g/L, and 43% respectively. The extracted lipids exhibited similarities to those of vegetable oils. The carbon chain length of accumulated fatty acids ranged from C10 to C21, mainly oleic acid (31%). Moreover, biodiesel properties based on lipid profile yielded cetane number (59.88), iodine value (42.29 mg I₂/g), high heating value (44.26 MJ/kg), and density (0.9 g/cm³) were in the permissible limits of international standards. The present study gives a sustainable biorefinery approach for the production of biodiesel from waste office paper hydrolysate.

有效利用废纸作为生物柴油生产的基材具有环境和经济优势。为研究利用废办公自动化纸生产微生物脂质的可行性，采用酶解法制备废纸水解物（WPH）。WPH 被喂给混浊红球菌PD630 用于微生物脂质生产。在测试的氮源中，含有NH _{4的培养基}Cl 分别产生了 4.92 和 1.29 g/L 的显着高生物量和脂质。当C/N比值从10增加到80时，脂质积累增加，脂质含量也从18.92%增加到43.41%。在优化条件下，获得的最大生物量、脂质产量和脂质含量分别为6.51 g/L、2.8 g/L和43%。提取的脂质表现出与植物油的相似性。累积脂肪酸的碳链长度范围为C10至C21，主要是油酸（31%）。此外，基于脂质分布的生物柴油特性产生十六烷值 (59.88)、碘值 (42.29 mg I ₂ /g)、高热值 (44.26 MJ/kg) 和密度 (0.9 g/cm ³) 在国际标准的允许范围内。本研究提供了一种可持续的生物精炼方法，用于从废办公纸水解物中生产生物柴油。