• The fruit surface has an enormous impact on the external appearance and postharvest shelf-life of fruit. Here, we report two functionally redundant genes, PpMYB25 and PpMYB26, involved in regulation of fruit skin texture in peach.
• PpMYB25 can activate transcription of PpMYB26 and they both induce trichome development and cuticular wax accumulation, resulting in peach fruit with a fuzzy and dull appearance. By contrast, nonfunctional mutation of PpMYB25 caused by an insertional retrotransposon in the last exon in nectarine fails to activate transcription of PpMYB26, resulting in nectarine fruit with a smooth and shiny appearance due to loss of trichome initiation and decreased cuticular wax accumulation.
• Secondary cell wall biosynthesis in peach fruit pubescence is controlled by a transcriptional regulatory network, including the master regulator PpNAC43 and its downstream MYB transcription factors such as PpMYB42, PpMYB46 and PpMYB83.
• Our results show that PpMYB25 and PpMYB26 coordinately regulate fruit pubescence and cuticular wax accumulation and their simultaneous perturbation results in the origin of nectarine, which is botanically classified as a subspecies of peach.

中文翻译：

---

两个R2R3-MYB基因协同控制桃花毛状体发育和表皮蜡生物合成

• 果实表面对果实的外观和采后保质期有着巨大的影响。在这里，我们报告了两个功能冗余基因PpMYB25和PpMYB26，它们参与调节桃果实的皮肤质地。
• PpMYB25可以激活PpMYB26的转录，它们都可以诱导毛状体发育和角质层蜡质积累，导致桃果实外观模糊、无光泽。相比之下，由油桃最后一个外显子中的插入逆转录转座子引起的PpMYB25非功能性突变未能激活PpMYB26的转录，导致由于毛状体起始丧失和表皮蜡积累减少而导致油桃果实具有光滑和有光泽的外观。
• 桃果实短毛中的次生细胞壁生物合成受转录调控网络控制，包括主调控因子 PpNAC43 及其下游 MYB 转录因子，如 PpMYB42、PpMYB46 和 PpMYB83。
• 我们的研究结果表明，PpMYB25和PpMYB26协同调节果实的短柔毛和表皮蜡的积累，并且它们的同时扰动导致油桃的起源，油桃在植物学上被归类为桃的一个亚种。