Using waste biomass as raw material for culture medium is an important way to lower the production cost of biodiesel. In this study, Scenedesmus obliquus was used as an oil-producing microalgae and culture medium was prepared from a cyanobacterial bloom by hydrothermal oxidation. The growth of S. obliquus was strongly inhibited by organic compounds with molecular weight >3 kDa. However, nutrients <3 kDa accounted for >85% of the total nutrition in the raw medium, and medium containing only low-molecular-weight organic matter (i.e., from which material >3 kDa was removed) promoted the growth of S. obliquus: the maximum dry weight of S. obliquus reached 2.61 g L⁻¹ and the logarithmic-phase specific growth rate 0.371 d⁻¹, while the oil content remained >14%. These data represent a considerable improvement in the biomass and total oil yields compared with growth in BG11 medium or the unfractionated raw medium prepared from the cyanobacterial slurry.

利用废弃生物质作为培养基原料是降低生物柴油生产成本的重要途径。在这项研究中，斜栅藻被用作产油微藻，并通过水热氧化从蓝藻水华制备培养基。S. obliquus的生长受到分子量>3 kDa 的有机化合物的强烈抑制。然而，<3 kDa 的营养成分占原料培养基总营养的 >85%，而仅含有低分子量有机物（即去除了 >3 kDa 的物质）的培养基促进了S. obliquus的生长： S. obliquus的最大干重达到2.61 g L ^-1对数期比生长速率为0.371 d ^-1，而含油量保持>14%。与在 BG11 培养基或由蓝藻浆液制备的未分级原料培养基中的生长相比，这些数据代表了生物量和总油产量的显着提高。