The current study aims to optimize in vitro elongation and rooting protocol and successful acclimatization of two elite date palm cultivars of Pakistan. The differentiated repeated embryos (RE) and non-repeated embryos (NRE) of date palm cvs. Aseel and Dhakki were harvested and transferred on germination medium containing different combinations of NAA + Kin (mg L⁻¹), full strength MS nutrients and 30 g L⁻¹ sucrose. The maximum somatic embryos germination and plantlet formation was achieved on the medium comprising 0.05 NAA + 1.0 Kin (mg L⁻¹). Whereas improved elongation and rooting was obtained on the MS medium comprising 0.1 NAA + 0.1 BA (mg L⁻¹). Addition of activated charcoal (3 g L⁻¹) and trimming of roots (prior to proper rooting stage on rooting medium) were found vital to enhance leaf and root length, in addition helped secondary and tertiary root formation. The in vitro hardening was observed as helpful to bring gradual physiological changes from heterotrophic to autotrophic mode of plant nutrition. In vitro plantlets with 2–4 leaves (avg. 20 cm long) and healthy roots (avg. 8–12 cm long) were acclimatized in greenhouse on different soil mixtures. The best soil medium for acclimatization of plantlets was peat moss + river sand mixture at ratio of 3:1 (v/v) which resulted in 92% survival of plantlets in the greenhouse during first month of transferring. The current micropropagation protocol may support for commercial scale production of other elite date palm cultivars worldwide.

目前的研究旨在优化巴基斯坦两个优良枣椰树品种的体外伸长和生根方案以及成功驯化。海枣cvs的分化的重复胚胎（RE）和非重复胚胎（NRE）。收获 Aseel 和 Dhakki 并将其转移到含有不同组合的 NAA + Kin (mg L ^-1 )、全强度 MS 营养素和 30 g L ^-1蔗糖的萌发培养基上。^{在包含0.05 NAA + 1.0 Kin (mg L -1} )的培养基上实现了最大的体细胞胚萌发和小植株形成。^{而在包含0.1 NAA + 0.1 BA (mg L -1} )的MS培养基上获得了改善的伸长率和生根。添加活性炭（3 g L ^-1) 和修剪根部（在生根培养基上适当的生根阶段之前）被发现对于增加叶和根的长度至关重要，此外还有助于二级和三级根的形成。观察到体外硬化有助于使植物营养从异养模式逐渐转变为自养模式。体外具有 2-4 片叶子（平均 20 厘米长）和健康根（平均 8-12 厘米长）的小植株在温室中适应不同的土壤混合物。幼苗驯化的最佳土壤培养基是泥炭藓 + 河沙混合物，比例为 3:1 (v/v)，在转移的第一个月，温室中 92% 的幼苗成活率。当前的微繁殖协议可能支持全球其他优良枣椰树品种的商业规模生产。