Abstract

Carbapenem-resistant Serratia marcescens (CRE-S. marcescens) has recently emerged as an opportunistic human pathogen that causes various nosocomial and respiratory tract infections. The prognosis for CRE-S. marcescens-related infections is very poor and these infections are difficult to treat. This study investigated the synthesis of tea tree oil nanoemulsion (TTO-NE) and its impact on CRE-S. marcescens both in vitro and in vivo. TTO-NE was characterized by dynamic light scattering (DLS) and effectively eradicated bacterial planktonic and sessile forms, reduced bacterial virulence factors, and generated reactive oxygen species (ROS) in the bacterial cell. Notably, TTO-NE was efficient in reducing the colonization of CRE-S. marcescens in a C. elegans in vivo model. The data suggest that TTO-NE might be an excellent tool to combat infections associated with CRE-S. marcescens.

摘要

耐碳青霉烯类粘质沙雷氏菌(CRE - S. marcescens ) 最近作为一种机会性人类病原体出现，可导致各种医院和呼吸道感染。CRE-粘质沙门氏菌相关感染的预后非常差，并且这些感染难以治疗。本研究调查了茶树油纳米乳液 (TTO-NE) 的合成及其对体外和体内CRE- S. marcescens的影响. TTO-NE 的特点是动态光散射 (DLS) 并有效根除细菌浮游和固着形式，减少细菌毒力因子，并在细菌细胞中产生活性氧 (ROS)。值得注意的是，TTO-NE 在减少秀丽隐杆线虫体内模型中粘质CRES-S.的定植方面是有效的。数据表明，TTO-NE 可能是对抗与 CRES- S. marcescens相关的感染的极好工具。