In this study, inter-simple sequence repeats (ISSR) markers were used to investigate genetic relationships of 83 mulberry (Morus alba L.) genotypes being located at 38° 39′ 45′ 40′ 50′N latitude and 45′ 40° 12′E longitude in Turkey. Eight primer combinations generated a total of 106 bands, 95 of which were polymorphic. The polymorphism rate of the primers was found to be 89.5% on average. The highest polymorphism rate was obtained from the primer no. 807 (100%) and lowest rate was obtained from the primer 841 (80%). The number of bands obtained per primer ranged from 7 (ISSR 807) to 18 (ISSR 835), and the mean band number was found to be 13.25. The mean genetic similarity coefficients among all mulberry genotypes ranged between 0.54 and 1.00. In genotypes, the highest genetic similarity value was found in TO25 and TO39 genotypes (1.00). An UPGMA cluster of the 83 mulberry genotypes constructed from ISSR fragment Nei’s similarities separated 83 mulberry genotypes into four major clusters groups. The results from the cluster analysis were in general agreement with our morphologic classification. In addition, the fact that genotypes have vast genetic variation in a narrow area showed that these genotypes can be used in future mulberry breeding programs.

本研究采用简单序列重复序列间(ISSR)标记对83株桑树( Morus alba)的亲缘关系进行调查。 L.) 基因型位于土耳其北纬 38° 39' 45' 40' 50' 和东经 45' 40° 12'。八种引物组合共产生 106 个条带，其中 95 个是多态性的。引物的多态性平均为89.5%。从引物号获得最高的多态性。807 (100%) 和最低比率是从引物 841 (80%) 获得的。每个引物获得的条带数从 7 (ISSR 807) 到 18 (ISSR 835) 不等，平均条带数为 13.25。所有桑树基因型之间的平均遗传相似系数在0.54和1.00之间。在基因型中，最高的遗传相似性值出现在 TO25 和 TO39 基因型中（1.00）。由 ISSR 片段 Nei 的相似性构建的 83 种桑树基因型的 UPGMA 聚类将 83 种桑树基因型分成四个主要聚类组。聚类分析的结果与我们的形态学分类基本一致。此外，基因型在狭窄区域内具有巨大遗传变异的事实表明，这些基因型可用于未来的桑树育种计划。