The aim of this study was to explain differences in the biological behaviour of two boron carbide powders prepared by different synthesis methods. The commercial powder HD had a lower boron content in comparison to the synthesized powder SP. The analysis both powders surface confirmed the higher content of B-C bonds but the presence of heteroatomic B-C-O bonds indicating the oxide passivation of the surface of particles of only HD powder. On the other hand, the SP powder contains more boron in the volume as well as on the surface of particles. These differences facilitated the interaction between the carbide particles with the proteins in the culture medium. Proteins from the culture medium and active collagen peptides were more easily adsorbed to the surface of the synthesized powder particles, which was evidenced by new bands appearing in the spectrum. This translated into an increasing hydrophilicity of the synthesized boron carbide powder. The presence of proteins on the surface of the powder facilitated the cells adhesion of macrophages and fibroblasts. The synthesized boron carbide powder improved the macrophage functional status; the cells were characterized by high viability and low cytotoxicity as well as the correct morphology.

本研究的目的是解释通过不同合成方法制备的两种碳化硼粉末的生物学行为差异。与合成粉末 SP 相比，商业粉末 HD 具有较低的硼含量。两种粉末表面的分析证实了较高含量的 BC 键，但杂原子 BCO 键的存在表明仅 HD 粉末颗粒表面的氧化物钝化。另一方面，SP 粉末在体积和颗粒表面上含有更多的硼。这些差异促进了碳化物颗粒与培养基中蛋白质之间的相互作用。培养基中的蛋白质和活性胶原蛋白肽更容易吸附到合成粉末颗粒的表面，频谱中出现的新频段证明了这一点。这转化为合成的碳化硼粉末的亲水性增加。粉末表面蛋白质的存在促进了巨噬细胞和成纤维细胞的细胞粘附。合成的碳化硼粉末改善了巨噬细胞的功能状态；细胞的特点是高活力和低细胞毒性以及正确的形态。