MYB transcription factors, one of the largest gene families in plants, play an important role in plant stress response. In this paper, MYBs of melon were identified and classified by genome wide analysis, and their physicochemical properties, structural characteristics and phylogenetic relationships were analyzed. The response of melon MYB to autotoxicity and saline-alkali stress was analyzed by using transcriptome sequencing database and RT-qPCR. A total of 178 MYBs was identified from melon genome database, which can be divided into four subfamilies, of which 81 belong to 1R-MYB subfamily and 95 belong to R2R3-MYB subfamily. 2R-MYB subfamily is more conservative than 1R-MYB subfamily. Most MYB were located in the nucleus. Only a few members were located in chloroplast, cytoplasm, plastid and mitochondria. MYBs were distributed on all 12 chromosomes of melon, with 27 on chromosome 4 and 6 on chromosome 10. All of the 12 chromosomes contained duplicated MYB genes, whereas chromosomes 4 contained the highest number, 10. Tandem and fragment duplication play an important role in the evolution of melon MYB genes. The Cis-acting element of melon MYB contain three types of response elements: defense and stress response elements (DSREs), light response elements (LREs) and hormone response elements (HREs). 60 melon MYBs were differentially expressed under autotoxicity, 6 were involved in saline alkali response, CmMYB2R40 and CmMYB2R54 were both responsive to autotoxicity and saline-alkali stress. MYBs, which involved in the response of melon autotoxicity and saline-alkali stress, were closely related to osmotic regulation, stomatal on–off, and autotoxicity chemicals synthesis. The results of this paper will contribute to the comprehensive understanding of the characteristics of MYBs in melon and its potential role in stress resistance.

MYB转录因子是植物中最大的基因家族之一，在植物应激反应中发挥着重要作用。本文通过全基因组分析对甜瓜的MYBs进行鉴定和分类，分析其理化性质、结构特征和系统发育关系。使用转录组测序数据库和RT-qPCR分析了甜瓜MYB对自身毒性和盐碱胁迫的反应。总计 178 MYBs从甜瓜基因组数据库中鉴定，可分为4个亚科，其中81个属于1R-MYB亚科，95个属于R2R3-MYB亚科。2R-MYB 亚科比 1R-MYB 亚科更保守。大多数MYB位于细胞核中。只有少数成员位于叶绿体、细胞质、质体和线粒体中。MYBs分布在甜瓜的全部 12 条染色体上，其中 27 条在 4 号染色体上，6 号在 10 号染色体上。所有 12 条染色体都含有重复的MYB基因，而 4 号染色体含有最多的数量，即 10。串联和片段重复在甜瓜中起重要作用。甜瓜MYB基因的进化。甜瓜MYB的顺式作用元件包含三种类型的反应元件：防御和压力反应元件（DSREs）、光反应元件（LREs）和激素反应元件（HREs）。60个甜瓜MYB在自毒作用下差异表达，6个参与盐碱反应，CmMYB2R40和CmMYB2R54均对自毒和盐碱胁迫有反应。MYBs参与了甜瓜自毒和盐碱胁迫的响应，与渗透调节、气孔开关和自毒化学物质的合成密切相关。本文的研究结果将有助于全面了解甜瓜中MYBs的特征及其在抗逆性中的潜在作用。