Vegetable grafting has become an important method for developing resistance to biotic and abiotic stresses and increasing potential yield in agronomic practice. To determine the effects of grafting on tomato taste, we examined the cultivated tomato ‘Moneymaker’ and the wild tomato Solanum pimpinellifolium, which have different fruit weights and qualities, to investigate the effect of grafting on sugars, acids, and volatiles in single-head and double-head grafted plants using GC-MS and LC-MS. We observed that the contents of sugars, acids and volatiles in heterografted tomato pericarps are influenced by rootstocks. Different metabolites exhibit distinct responses to the rootstock and might be associated with rootstock-scion interactions. Comparison of the metabolites in the heterogeneous fruit of the single- and double-head grafted plants indicates that the grafting method also influences the metabolic changes in grafted plants. Moreover, we also identified numerous mobile transcripts and determined that the mobile mRNAs are associated with metabolic processes in tomato leaves. Our work helps to elucidate the effect of genotype and graft method on fruit quality and may provide a basis for future research on rootstock breeding and selection in plants.

在农艺实践中，蔬菜嫁接已成为发展对生物和非生物胁迫的抗性和增加潜在产量的重要方法。为了确定嫁接对番茄味道的影响，我们检查了栽培番茄“Moneymaker”和野生番茄Solanum pimpinellifolium, 使用 GC-MS 和 LC-MS 研究嫁接对单头和双头嫁接植物中糖、酸和挥发物的影响。我们观察到异种嫁接番茄果皮中糖、酸和挥发物的含量受砧木的影响。不同的代谢物对砧木表现出不同的反应，可能与砧木-接穗的相互作用有关。单头和双头嫁接植物异质果实中代谢物的比较表明嫁接方法也影响嫁接植物的代谢变化。此外，我们还鉴定了许多移动转录本，并确定移动 mRNA 与番茄叶片中的代谢过程有关。