Microalgae are known to produce neutral-lipids such as triacylglycerols (TAGs), a raw material required for biofuel production. The present study aimed to screen the high lipid producing native microalgae strains from freshwater habitats, select appropriate methods to extract lipid from wet algal biomass, and study fatty acid compositions. At first, isolated twenty native strains among them two isolates that exhibited higher lipid content was further subjected to molecular characterization. Results based on the cell morphology, molecular characterization, and phylogenetic analysis indicated that these two strains were Tetradesmus obliquus and Ettlia oleoabundans. Based on the growth study of screened algal strains, the biomass ranged from 0.65 g/L to 6.03 g/L with Tetradesmus obliquus providing the highest biomass and total lipid content of 51% when cultured in a nitrogen-deprived medium. The highest lipid yield was obtained with hexane:isopropanol (2:1) solvent mixtures, accompanied by an optimized cell wall disruption method. Additionally, it was found that Tetradesmus obliquus had higher contents of saturated and monounsaturated fatty acids, i.e., 36.19% and 31.49%, respectively, in nitrogen-deprived medium (N⁻), whereas in nitrogen-containing medium (N⁺) was 27.34% and 28.85%, respectively. Hence, this suggests their suitability for biofuel production.

众所周知，微藻会产生中性脂质，例如三酰甘油 (TAG)，这是生产生物燃料所需的原材料。本研究旨在从淡水栖息地筛选高产脂质的天然微藻菌株，选择合适的方法从湿藻生物质中提取脂质，并研究脂肪酸组成。首先，分离出 20 种天然菌株，其中两种具有较高脂质含量的分离株被进一步进行分子表征。基于细胞形态、分子特征和系统发育分析的结果表明，这两个菌株是Tetradesmusobliquus和Ettlia oleoabundans。根据筛选的藻类菌株的生长研究，生物量范围为 0.65 g/L 至 6.03 g/L，其中在缺氮培养基中培养时，斜纹石斛提供最高的生物量和 51% 的总脂质含量。使用己烷: 异丙醇 (2:1) 溶剂混合物获得最高的脂质产量，同时采用优化的细胞壁破坏方法。此外，研究发现，在缺氮培养基 (N ^- ) 中，斜纹石斛的饱和脂肪酸和单不饱和脂肪酸含量较高，分别为 36.19% 和 31.49% ，而在含氮培养基 (N ⁺ ) 中为 27.34 % 和 28.85%，分别。因此，这表明它们适用于生物燃料生产。