Development of biosensing systems resembling optical 96-well plates using portable single-channel electrochemical analyzers is usually a great challenge. Herein, a light-addressable paper-based photoelectrochemical (PEC) analytical device suitable for on-site high-throughput biosensing is reported. This device consists of a solar cell-type single-channel PEC system with plenty of separated detection zones. Each zone contains a silver nanowires/fullerene-Congo red (AgNWs/C₆₀-CR) disc working electrode and a AgNWs ring reference/counter electrode, which can be massively produced by a simple filtration and laser cutting method. Taking advantage of the sensitive photocurrent response of thiocholine (TCl) on AgNWs/C₆₀-CR, an acetylcholinesterase (AChE)-based PEC biosensing system with tunable detection throughput for the on-site screening of ultratrace organophosphorus pesticides (OPs) was established.

中文翻译：

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用于现场生物传感的具有可调检测通量的光寻址纸基光电化学分析装置

使用便携式单通道电化学分析仪开发类似于光学 96 孔板的生物传感系统通常是一个巨大的挑战。本文报道了一种适用于现场高通量生物传感的光寻址纸基光电化学 (PEC) 分析装置。该设备由一个太阳能电池型单通道 PEC 系统组成，具有大量分离的检测区域。每个区域包含一个银纳米线/富勒烯-刚果红 (AgNWs/C ₆₀ -CR) 圆盘工作电极和一个 AgNWs 环参考/对电极，可以通过简单的过滤和激光切割方法大量生产。利用硫代胆碱 (TCl) 在 AgNWs/C _{60上的灵敏光电流响应}-CR 是一种基于乙酰胆碱酯酶 (AChE) 的 PEC 生物传感系统，具有可调节的检测通量，用于现场筛选超痕量有机磷农药 (OPs)。