Mammalian cells are widely used for producing recombinant glycoproteins of pharmaceutical interest. However, a major drawback of using mammalian cells is the high production costs associated with uniformly isotope-labeled glycoproteins due to the large quantity of labeled l-glutamine required for their growth. To address this problem, we developed a cost-saving method for uniform isotope labeling by cultivating the mammalian cells under glutamine-free conditions, which was achieved by co-expression of glutamine synthase. We demonstrate the utility of this approach using fucosylated and non-fucosylated Fc glycoforms of human immunoglobulin G1.

哺乳动物细胞广泛用于生产具有药学意义的重组糖蛋白。然而，使用哺乳动物细胞的一个主要缺点是与统一同位素标记的糖蛋白相关的高生产成本，因为它们的生长需要大量标记的l-谷氨酰胺。为了解决这个问题，我们开发了一种通过在无谷氨酰胺条件下培养哺乳动物细胞来进行统一同位素标记的节省成本的方法，这是通过谷氨酰胺合酶的共表达来实现的。我们使用人免疫球蛋白 G1 的岩藻糖基化和非岩藻糖基化 Fc 糖型证明了这种方法的实用性。