Pyramiding Rpp genes is a promising strategy to achieve a more durable resistance against Asian soybean rust (ASR). The objective of this study was to develop Rpp-pyramided populations and evaluate their resistance and yield in field conditions. Fifteen pyramided populations carrying different combinations of Rpp genes were developed, and the F₃ lines were evaluated to phenotypic resistance and yield performance. Our results suggest that the Rpp1-b of different resistant sources (PI 587880A, PI 594723, PI 4538A) have higher resistance and yield performance compared with other Rpp genes. Rpp-pyramided populations carrying two and three genes showed an increase in resistance compared with populations carrying a single Rpp gene. The best gene combinations were Rpp1-b + Rpp5, showing neither uredinia formation nor sporulation (immunity + RB1 phenotype reaction) in more than 70% of the F₃ population. Rpp-pyramided lines developed in this study have the potential to be used in backcross programs for improving ASR resistance.

中文翻译：

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基因金字塔组合赋予亚洲大豆锈病抗性

Pyramiding Rpp基因是实现对亚洲大豆锈病 (ASR) 的更持久抗性的有前景的策略。本研究的目的是开发Rpp -pyramided 种群并评估它们在田间条件下的抗性和产量。开发了 15 个携带不同Rpp基因组合的金字塔种群，并评估了 F ₃系的表型抗性和产量性能。我们的研究结果表明，与其他Rpp基因相比，不同抗性来源（PI 587880A、PI 594723、PI 4538A）的Rpp1-b具有更高的抗性和产量性能。Rpp与携带单个Rpp基因的群体相比，携带两个和三个基因的 -pyramided 群体显示出抗性增加。最好的基因组合是Rpp1-b + Rpp5，在超过 70% 的 F ₃种群中既没有显示尿素形成也没有孢子形成（免疫 + RB1 表型反应）。本研究中开发的Rpp- pyramided 系有可能用于回交程序以提高 ASR 抗性。