Architecture of torovirus replicative organelles,Molecular Microbiology

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Architecture of torovirus replicative organelles
Molecular Microbiology ( IF 2.6 ) Pub Date : 2021-12-30 , DOI: 10.1111/mmi.14875
Ginés Ávila-Pérez ₁ , María Teresa Rejas ₂ , Francisco Javier Chichón ₃ , Milagros Guerra ₂ , José Jesús Fernández ₄ , Dolores Rodríguez ₁

Affiliation

Plus-stranded RNA viruses replicate in the cytosol of infected cells, in membrane-bound replication complexes. We previously identified double membrane vesicles (DMVs) in the cytoplasm of cells infected with Berne virus (BEV), the prototype member of the Torovirus genus (Nidovirales Order). Our previous analysis by transmission electron microscopy suggested that the DMVs form a reticulovesicular network (RVN) analogous those described for the related severe acute respiratory syndrome coronavirus (SARS-CoV-1). Here, we used serial sectioning and electron tomography to characterize the architecture of torovirus replication organelles, and to learn about their biogenesis and dynamics during the infection. The formation of a RVN in BEV infected cells was confirmed, where the outer membranes of the DMVs are interconnected with each other and with the ER. Paired or zippered ER membranes connected with the DMVs were also observed, and likely represent early structures that evolve to give rise to DMVs. Also, paired membranes forming small spherule-like invaginations were observed at late time post-infection. Although resembling in size, the tomographic analysis show that these structures are clearly different from the true spherules described previously for coronaviruses. Hence, BEV shows important similarities, but also some differences, in the architecture of the replication organelles with other nidoviruses.

中文翻译：

环病毒复制细胞器的结构

正链 RNA 病毒在受感染细胞的胞质溶胶中以膜结合复制复合物的形式复制。我们之前在感染伯尔尼病毒 (BEV) 的细胞的细胞质中发现了双膜囊泡 (DMV)，伯尔尼病毒 (BEV) 是环病毒属 ( Nidovirales ) 的原型成员命令）。我们之前通过透射电子显微镜进行的分析表明，DMV 形成了一个网状水泡网络（RVN），类似于相关的严重急性呼吸综合征冠状病毒（SARS-CoV-1）所描述的那些。在这里，我们使用连续切片和电子断层扫描来表征环病毒复制细胞器的结构，并了解它们在感染过程中的生物发生和动力学。证实了在 BEV 感染的细胞中形成了 RVN，其中 DMV 的外膜相互连接并与 ER 连接。还观察到与 DMV 连接的成对或带拉链的 ER 膜，并且可能代表进化为产生 DMV 的早期结构。此外，在感染后后期观察到成对的膜形成小球状内陷。尽管大小相似，但断层扫描分析表明，这些结构明显不同于之前描述的冠状病毒的真实小球。因此，BEV 在复制细胞器与其他巢状病毒的结构中显示出重要的相似性，但也存在一些差异。

更新日期：2021-12-30

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