Today, investigating the adverse effects of nanoparticles is one of the challenges that researchers are facing. In this study, Al₂W₃O₁₂ nanoparticles (AWO NPs) were prepared using the ultrasonic procedure for the first time. The probable mechanism for ultrasonic synthesis is creating high-temperature bubbles and nanoreactors in the synthesis medium. Therefore, in this method, the reaction rate and efficiency are improved. An optimum sample in terms of size and morphology was selected by applying multiple analysis such as X-ray diffraction (XRD), Transmission electron microscope (TEM), and Scanning electron microscopy (SEM). After that, three different concentrations of NPs (40, 80, 160 ppm) were added to the algal culture medium to investigate the effect of NPs on algae growth. After ten days, biological surveys, including the amount of biomass, number of cells, carotenoids, chlorophyll-a, chlorophyll-b, and malondialdehyde (MDA), were measured. The results showed that a concentration of 80 ppm could be used to increase the growth rate of algae for biotechnology purposes. Furthermore, using higher concentrations in cases involving the aquatic environment could be dangerous. These achievements can be of great importance for mass production and biotechnological exploitation of such algae.

今天，研究纳米粒子的不利影响是研究人员面临的挑战之一。在这项研究中，Al ₂ W ₃ O ₁₂纳米粒子（AWO NPs）首次使用超声程序制备。超声波合成的可能机制是在合成介质中产生高温气泡和纳米反应器。因此，在该方法中，提高了反应速率和效率。通过应用 X 射线衍射 (XRD)、透射电子显微镜 (TEM) 和扫描电子显微镜 (SEM) 等多种分析方法选择尺寸和形态方面的最佳样品。之后，将三种不同浓度的 NPs（40、80、160 ppm）添加到藻类培养基中，以研究 NPs 对藻类生长的影响。十天后，生物调查，包括生物质的量，细胞，类胡萝卜素的数目，叶绿素一个，叶绿素b和丙二醛 (MDA) 进行了测量。结果表明，80 ppm 的浓度可用于提高用于生物技术目的的藻类的生长速度。此外，在涉及水生环境的情况下使用更高的浓度可能是危险的。这些成就对于此类藻类的大规模生产和生物技术开发具有重要意义。