In this study, CS-NeuAc hydrogel was prepared from chitosan (CS) and N-acetylneuraminic acid (NeuAc) using cyclic freeze/thaw under 1-ethyl-3-(3-dimethylaminopropyl)-carbodiiminde/N-hydroxysuccinimide catalysis. Fourier transformed infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses confirmed the successful synthesis of CS-NeuAc hydrogel. CS-NeuAc hydrogels were found to be equipped with suitable swelling behaviour, porosity, water evaporation rate, degradation rate, and good mechanical properties compared to CS. The swelling rate, water evaporation rate, and degradation rate of prepared hydrogel with a CS/NeuAc molar ratio of 10:1 were 710.7 ± 28.6%, 78.2 ± 1.6%, and 58.6 ± 1.5%, respectively. Moreover, CS-NeuAc hydrogel exhibited excellent biocompatibility. The evaluation of CS-NeuAc hydrogels’ antibacterial activity confirmed that they serve as effective antibacterial materials against Escherichia coli and Staphylococcus aureus with antibacterial rates above 95% and 92%, respectively, and the minimum inhibitory concentrations was above 400 μg/ml. In conclusion, CS-NeuAc hydrogel can be a candidate for biological antibacterial materials.

在这项研究中，CS-NeuAc 水凝胶是由壳聚糖 (CS) 和 N-乙酰神经氨酸 (NeuAc) 在 1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺/N-羟基琥珀酰亚胺催化下使用循环冷冻/解冻制备的。傅里叶变换红外光谱、X 射线衍射和扫描电子显微镜分析证实了 CS-NeuAc 水凝胶的成功合成。与 CS 相比，CS-NeuAc 水凝胶具有合适的溶胀行为、孔隙率、水分蒸发速率、降解速率和良好的机械性能。制备的 CS/NeuAc 摩尔比为 10:1 的水凝胶的溶胀率、水分蒸发率和降解率分别为 710.7±28.6%、78.2±1.6% 和 58.6±1.5%。此外，CS-NeuAc 水凝胶表现出优异的生物相容性。大肠杆菌和金黄色葡萄球菌的抑菌率分别在95%和92%以上，最低抑菌浓度在400 μg/ml以上。总之，CS-NeuAc水凝胶可以成为生物抗菌材料的候选者。