Many surgical metals such as stainless steel, titanium, magnesium and its alloys have been extensively used for the recovery of body structures in human beings. Corrosion is the major reason for failure in metallic implants, when the metal comes in contact with the body fluids it releases metal ions into the surrounding tissues. This may even lead to the second surgery which can be eradicated by the surface modification of the implant with bioceramics using coating techniques. The present work involves the development of coatings on the surface of 316L SS type of stainless steel using a biphasic mixture of bio ceramics HAP/β-TCP in ratio of 7.5:2.5 by electrophoretic deposition (EPD) from a suspension of ethanol. The presence of biphasic coating imparts the property of both bioactivity and bioresorbability to the implant with good adherence of the coatings in body fluids. These coatings provide corrosion resistance and also favour new bone growth. Further, the biocompatibility of these materials can be evaluated by in-vitro assay. This includes cytotoxicity tests carried out with normal cell line (Vero cell line) and cancerous cell line (HEP II cell Line). The coated samples have been tested for their biochemical nature using DPPH (2,2 diphenyl 1 picryl hydrazil compound) activity to confirm whether the coated implant is suitable for cancerous patients with its antioxidant property which helps to trap the free radicals.

中文翻译：

---

用于骨科应用的生物陶瓷涂层材料的评估

许多外科金属，如不锈钢、钛​​、镁及其合金，已被广泛用于人体结构的恢复。腐蚀是金属植入物失败的主要原因，当金属与体液接触时，它会将金属离子释放到周围组织中。这甚至可能导致第二次手术，这可以通过使用涂层技术用生物陶瓷对植入物进行表面改性来根除。目前的工作涉及使用比例为 7.5:2.5 的生物陶瓷 HAP/β-TCP 的双相混合物通过乙醇悬浮液的电泳沉积 (EPD) 在 316L SS 型不锈钢表面上开发涂层。双相涂层的存在赋予植入物生物活性和生物再吸收性的特性，同时涂层在体液中具有良好的粘附性。这些涂层提供耐腐蚀性，也有利于新骨的生长。此外，这些材料的生物相容性可以通过体外测定进行评估。这包括对正常细胞系（Vero 细胞系）和癌细胞系（HEP II 细胞系）进行的细胞毒性测试。已使用 DPPH（2,2 二苯基 1 苦基肼化合物）活性测试涂层样品的生化性质，以确认涂层植入物是否适合癌症患者，其抗氧化特性有助于捕获自由基。这些材料的生物相容性可以通过体外试验进行评估。这包括对正常细胞系（Vero 细胞系）和癌细胞系（HEP II 细胞系）进行的细胞毒性测试。已使用 DPPH（2,2 二苯基 1 苦基肼化合物）活性测试涂层样品的生化性质，以确认涂层植入物是否适合癌症患者，其抗氧化特性有助于捕获自由基。这些材料的生物相容性可以通过体外试验进行评估。这包括对正常细胞系（Vero 细胞系）和癌细胞系（HEP II 细胞系）进行的细胞毒性测试。已使用 DPPH（2,2 二苯基 1 苦基肼化合物）活性测试涂层样品的生化性质，以确认涂层植入物是否适合癌症患者，其抗氧化特性有助于捕获自由基。