Isolation of a protein mixture having IC50 of 60µg/mL against Streptococcus mutans from the methanol extract of bark of Manilkara hexandra (Roxb) along with six plants phenols Catechin, Catecol, Gallic acid, Phloroglucinol, Quercetin and Rutin has been reported. Where in phenolic constituents of the bark has been reported for the first time and the isolated compounds having moderate activity against S.mutans.In this work, the anti-oxidant activity of these pure compounds and fractions has been studied and its relevance in oral care is established. Comparative anti-oxidant activity of all the six compounds along with the crude extract and fractions have been assessed using eight different assay protocols. The results indicate that twice daily tooth cleaning with the bark can provide basic oral disease protection and thereby assuring several health benefits. Synergistic activity of the phenolic constituents along with the protein and other constituentsmight be a reasonable scientific explanation for the folklore use of the plant as one of the prescription for oral diseases.

中文翻译：

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Manilkara hexandra (Roxb) 树皮的化合物和组分的抗氧化活性

据报道，从 Manilkara hexandra (Roxb) 树皮的甲醇提取物以及六种植物酚类儿茶素、儿茶酚、没食子酸、间苯三酚、槲皮素和芦丁中分离出一种蛋白质混合物，其对变形链球菌的 IC50 为 60μg/mL。首次报道了树皮的酚类成分和对 S.mutans 具有中等活性的分离化合物。在这项工作中，研究了这些纯化合物和级分的抗氧化活性及其在口腔护理中的相关性成立。使用八种不同的测定方案评估了所有六种化合物以及粗提取物和级分的抗氧化活性比较。结果表明，每天两次用树皮清洁牙齿可以提供基本的口腔疾病保护，从而确保多种健康益处。酚类成分与蛋白质和其他成分的协同活性可能是民间传说将该植物用作口腔疾病处方之一的合理科学解释。