A fed-batch ABE fermentation process coupled with in-situ gas-stripping to mitigate butanol toxicity was investigated. Two feeding strategies were compared: pulses of sugars and continuous feeding of the liquid released in the dilute acid pretreatment of brewer's spent grain. The concentrations of butanol (13.2 g L^-1 total and 50 g L^-1 average in the condensates for glucose pulse feeding) were higher than those obtained under batch conditions, showing that in-situ gas-stripping can relieve butanol toxicity. The continuous feeding of the fed-batch reactor produced similar butanol concentrations (10.2 g L^-1 total and 65 g L^-1 in the condensates), more stable concentrations of solvents in the condensates and enhanced monosaccharides uptake (99.1%) in comparison with the pulse feeding strategy. The efficient utilization of the enzymatic and pretreatment hydrolysates in the same fed-batch reactor is an integrated approach that could reduce capital and operating costs. The mathematical model proposed showed good performance to predict concentrations in the fermentation broth.

研究了分批补料 ABE 发酵工艺与原位气提相结合以减轻丁醇毒性。比较了两种进料策略：糖脉冲和连续进料在啤酒废谷物的稀酸预处理中释放的液体。丁醇的浓度（葡萄糖脉冲进料的冷凝物中总共13.2 g L ^-1和平均50 g L ^-1）高于在批次条件下获得的浓度，表明原位气提可以减轻丁醇的毒性。分批补料反应器的连续进料产生了相似的丁醇浓度（总共10.2 g L ^-1和 65 g L ^-1在冷凝物中），与脉冲进料策略相比，冷凝物中的溶剂浓度更稳定，单糖吸收率更高（99.1%）。在同一个补料分批反应器中有效利用酶促和预处理水解物是一种可以降低资本和运营成本的综合方法。提出的数学模型在预测发酵液中的浓度方面表现出良好的性能。