Homochiral metal-organic frameworks (MOFs) have attracted considerable attention in many fields of research, such as chiral catalysis and chiral chromatography. However, only few homochiral MOFs can be effectively used in capillary electrochromatography (CEC) and their performances are far from adequate. In this study, we successfully synthesized achiral nanocrystalline MIL-53. A facile post-synthetic modification strategy was then implemented to functionalize the product, yielding a homochiral MOF: l-His-NH-MIL-53. This MOF was then employed as a chiral coating in open-tubular CEC mode (OT-CEC), and, as such, it exhibited high enantioselectivities for several racemic drugs. The homochiral MOF and the fabricated capillary coating were systematically characterized using transmission electron microscopy, scanning electron microscopy (with energy-dispersive X-ray spectrometry), Fourier-transform infrared spectroscopy, X-ray diffractometry, thermogravimetric analysis, circular dichroism spectroscopy, Brunauer-Emmett-Teller surface area measurements, and X-ray photoelectron spectroscopy. This study is expected to provide a new strategy for the design and establishment of MOF-based chiral OT-CEC systems.

单手性金属有机框架（MOFs）在手性催化和手性色谱等许多研究领域引起了广泛关注。然而，只有少数纯手性 MOF 可以有效地用于毛细管电色谱 (CEC)，其性能还远远不够。在这项研究中，我们成功合成了非手性纳米晶 MIL-53。然后实施一种简便的合成后修饰策略来对产品进行功能化，产生纯手性 MOF：l-His-NH-MIL-53。然后将该 MOF 用作开管 CEC 模式 (OT-CEC) 的手性涂层，因此，它对几种外消旋药物表现出高对映选择性。使用透射电子显微镜、扫描电子显微镜（具有能量色散 X 射线光谱）、傅里叶变换红外光谱、X 射线衍射、热重分析、圆二色光谱、Brunauer-系统地表征纯手性 MOF 和制造的毛细管涂层。 Emmett-Teller 表面积测量和 X 射线光电子能谱。该研究有望为基于 MOF 的手性 OT-CEC 系统的设计和建立提供新的策略。