ABSTRACT

Tebuconazole (TEB) is one of the most commonly used fungicides in the Lake Van Basin in the Eastern Anatolia Region, Turkey, which is one of the largest alkaline lakes in the world. In this study, the effects of TEB on the Van fish, the only vertebrate species living in Lake Van were investigated. The TEB was administered at a dose of 2.5 mg/L to the Van fish, which were examined histologically via the gill, liver, and kidney tissues after 24, 48, 72 and 96 h of exposure to TEB. Normal histological structure was observed in the gill, liver, and kidney tissues in the control group. Epithelial hyperplasia, epithelial necrosis, epithelial lifting, lamellar fusion, and an increase in mucus cell counts were observed in the gill tissues of the fish exposed to TEB. Dilatation and congestion of the sinusoids, melano-macrophages aggregation, and, focal necrosis were detected in the liver tissues of the fish exposed to TEB. Bleeding in the kidney tissues, hyaline droplet degeneration, contraction of the glomerulus and enlargement of the space within the Bowman’s capsule were observed. The findings of the present study indicated that sublethal concentrations of TEB result in histopathologic changes in the gill, liver, and kidney.

摘要

戊唑醇（TEB）是土耳其东部安纳托利亚地区凡湖盆地最常用的杀菌剂之一，该盆地是世界上最大的碱性湖泊之一。在这项研究中，研究了 TEB 对范鱼的影响，范鱼是唯一生活在范湖的脊椎动物物种。将 TEB 以 2.5 mg/L 的剂量施用于 Van 鱼，在暴露于 TEB 24、48、72 和 96 小时后，通过鳃、肝脏和肾脏组织对其进行组织学检查。对照组的鳃、肝、肾组织组织学结构正常。在暴露于TEB的鱼的鳃组织中观察到上皮增生、上皮坏死、上皮提升、层状融合和粘液细胞计数增加。血窦扩张和充血，黑色素巨噬细胞聚集，以及，在暴露于 TEB 的鱼的肝组织中检测到局灶性坏死。观察到肾组织出血、透明液滴变性、肾小球收缩和鲍曼氏囊内空间扩大。本研究的结果表明，亚致死浓度的 TEB 会导致鳃、肝脏和肾脏的组织病理学变化。