Sesquiterpene valencene is dominant in flavedo tissues of sweet oranges and imparts a unique woody aroma. However, the interaction between the biosynthetic pathways of valencene and other nutritional compounds is less studied. Sesquiterpenoids were significantly accumulated in a previously reported glossy mutant of orange (MT) than the wild type (WT), especially valencene and caryophyllene. In addition, we identified several other pathways with variations at both the transcriptional and metabolic levels in MT. It's interesting to found those up-regulated metabolites in MT, such as eukaryotic lipids, kaempferol and proline also showed strong positive correlation with valencene along with fruit maturation while those down-regulated metabolites, such as phenylpropanoid coumarins and most of the modified flavonoids exhibited negative correlation. We then categorized these shifted pathways into the ‘sesquitepenoid-identical shunt’ and the sesquitepenoid-opposite shunt’ and confirmed the classification result at transcriptional level. Our results provide important insights into the connections between various fruit quality-related properties.

倍半萜烯瓦伦烯在甜橙的flavedo 组织中占主导地位，并赋予独特的木质香气。然而，对瓦伦烯的生物合成途径与其他营养化合物之间的相互作用的研究较少。倍半萜类化合物在先前报道的橙色 (MT) 光泽突变体中比野生型 (WT) 显着积累，尤其是瓦伦烯和石竹烯。此外，我们确定了其他几种在 MT 转录和代谢水平上都有变化的途径。有趣的是，MT中上调的代谢物，如真核脂质、山柰酚和脯氨酸，随着果实成熟也与瓦伦烯呈强正相关，而那些下调的代谢物，如苯丙素香豆素和大多数改性黄酮类化合物则呈负相关。相关性。然后，我们将这些转移的途径分类为“倍半萜类相同分流”和倍半萜类相反分流，并在转录水平上确认分类结果。我们的结果为各种水果质量相关特性之间的联系提供了重要的见解。