ABSTRACT

Background, plasma-activated water (PAW) is generated by exposing water to the fourth state of matter (plasma). Plasma emits several kinds of reactive nitrogen and oxygen species (RNOS). This wealth of active species potentiates the PAW modulation of gamma radiation-induced oxidative stress, which is the most likely cause for DNA damaging effect and/or cell death following gamma irradiation. Aims, this study is an endeavor to investigate the role of oral administration of PAW, against high dose of gamma irradiation. Methods, tap water was exposed to plasma jet under consideration delivering a plasma dose of 0.425 J/cm². Rats, were divided into three groups, normal control ‘G1,’ group ‘G2’ including rats accessed to normal tap water and third group ‘G3’ including rats accessed to PAW. Each group drank the designated water for 60 consecutive days. After 60 days, the second and third groups were irradiated with a single dose of 7 gray gamma rays and anesthetized then sacrificed 24 following the irradiation. Blood samples were collected and assayed for DNA damage detection using comet assay. Biological functions including blood count, liver, and kidney function as well as oxygen and glucose levels were also assayed in the blood of all rats in the three considered groups. Results, although exposure to this dose of gamma radiation showed an adverse effects on rats accessed to normal tap water at both cellular and organ level, PAW group remained highly protected as was clearly shown by their cellular and biochemical parameters. Conclusions, our findings suggest that, PAW is considered a potentially promising technique for combating the acute effects of high radiation doses that damage DNA as well as impaired some biological functions. This could increase and support the hope in utilizing PAW as safer, economic, and efficient drinking water as radio-protective agent in expected wide scales.

摘要

背景，等离子体活化水 (PAW) 是通过将水暴露于第四种物质状态（等离子体）而产生的。等离子体会释放多种活性氮和氧物质 (RNOS)。这种丰富的活性物质增强了伽马辐射诱导的氧化应激的 PAW 调节，这是伽马辐射后 DNA 损伤效应和/或细胞死亡的最可能原因。目的，本研究旨在研究口服 PAW 对高剂量伽马辐射的作用。方法，考虑将自来水暴露于等离子射流，以提供 0.425 J/cm ²的等离子剂量. 将大鼠分成三组，正常对照组'G1'，'G2'组包括接触正常自来水的大鼠，第三组'G3'包括接触PAW的大鼠。每组连续饮用指定水60天。60 天后，第二组和第三组用单剂量的 7 次灰色伽马射线照射并麻醉，然后在照射后处死 24 只。收集血液样本并使用彗星试验分析 DNA 损伤检测。还在考虑的三个组的所有大鼠的血液中测定了生物学功能，包括血细胞计数、肝和肾功能以及氧和葡萄糖水平。结果，尽管暴露于这种剂量的伽马辐射对获得正常自来水的大鼠在细胞和器官水平上都显示出不利影响，PAW 组的细胞和生化参数清楚地表明，它们仍然受到高度保护。结论，我们的研究结果表明，PAW 被认为是一种具有潜在前景的技术，可用于对抗高辐射剂量的急性影响，这些辐射剂量会损害 DNA 并损害某些生物功能。这可以增加并支持将 PAW 作为更安全、经济和高效的饮用水作为辐射防护剂在预期的广泛范围内使用的希望。