The perennial Secale cereanum cultivar ‘Kriszta’ is an artificial hybrid of S. cereale and S. strictum ssp. anatolicum. From the cross between the wheat line Mv9kr1 and ‘Kriszta’, which aimed the transfer of beneficial traits from rye to wheat, numerous translocation lines have been produced. For the identification of the translocated chromosomes, the unambiguous differentiation between chromosome arms of ‘Kriszta’ is essential. The identification of its short chromosome arms using conventional FISH probes is easy, but because of their similar hybridization patterns, its long arms cannot be distinguished. The present study aimed to create the detailed karyotype of ‘Kriszta’, especially that of long arms, by both chromosome measurements and FISH using highly repetitive, as well as subtelomeric tandem repeat, and synthetic microsatellite DNA sequences. Our results indicate that the chromosome complement of ‘Kriszta’ is not a simple combination of the chromosomes of the parental rye species but is composed of rearranged chromosomes. It is also showed that an adequate pair-wise combination of the DNA sequences pSc119.2, pSc200, pSc250, and (AAC)₅ makes it possible to identify any of the long arms of S. cereanum cv. Kriszta chromosomes. The future usability of the identified wheat- ‘Kriszta’ translocated chromosomes is also discussed.

多年生黑麦草栽培品种“Kriszta”是S. creame和S.strictum ssp的人工杂交种。解剖学. 从小麦品系 Mv9kr1 和旨在将有益性状从黑麦转移到小麦的 'Kriszta' 之间的杂交，已经产生了许多易位品系。对于易位染色体的鉴定，'Kriszta' 染色体臂之间的明确区分是必不可少的。使用常规 FISH 探针识别其短染色体臂很容易，但由于它们的杂交模式相似，无法区分其长臂。本研究旨在通过染色体测量和 FISH 使用高度重复的亚端粒串联重复序列和合成微卫星 DNA 序列来创建“Kriszta”的详细核型，尤其是长臂的核型。我们的结果表明，'Kriszta' 的染色体补充不是亲本黑麦物种染色体的简单组合，而是由重新排列的染色体组成。还表明 DNA 序列 pSc119.2、pSc200、pSc250 和 (AAC) 的适当成对组合₅可以识别S. cereanum cv. 的任何长臂。克里斯塔染色体。还讨论了已鉴定的小麦-'Kriszta' 易位染色体的未来可用性。