Abstract

This study aimed to evaluate the antimicrobial and anti-biofilm activity of morin on polymicrobial biofilms and its cytotoxicity in controlled-release films and tablets based on gellan gum. Polymicrobial biofilms were formed from saliva for 48 h under an intermittent exposure regime to 1% sucrose and in contact with films or tablets of gellan gum containing 2 mg of morin each. Acidogenicity, bacterial viability, dry weight and insoluble extracellular polysaccharides from biofilms were evaluated. The cytotoxicity of morin was evaluated in oral keratinocytes. Morin released from the systems reduced the viability of all the microbial groups evaluated, as well as the dry weight and insoluble polysaccharide concentration in the matrix and promoted the control of acidogenicity when compared with the control group without the substance. Morin was cytotoxic only at the highest concentration evaluated. In conclusion, morin is an effective agent and shows antimicrobial and anti-biofilm activity against polymicrobial biofilms.

摘要

本研究旨在评估桑色素对多种微生物生物膜的抗菌和抗生物膜活性及其在基于结冷胶的控释膜和片剂中的细胞毒性。多微生物生物膜由唾液在 1% 蔗糖的间歇暴露条件下 48 小时形成，并与每片含有 2 mg 桑葚素的结冷胶薄膜或片剂接触。评估了来自生物膜的产酸性、细菌活力、干重和不溶性胞外多糖。在口腔角质形成细胞中评估了桑色素的细胞毒性。与没有该物质的对照组相比，从系统中释放的莫林降低了所有评估微生物组的活力，以及基质中的干重和不溶性多糖浓度，并促进了对酸原性的控制。Morin 仅在评估的最高浓度下具有细胞毒性。总之，桑色素是一种有效的药剂，对多种微生物生物膜显示出抗菌和抗生物膜活性。