Design and engineering of neuroglobin to catalyze the synthesis of indigo and derivatives for textile dyeing,Molecular Systems Design & Engineering

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Design and engineering of neuroglobin to catalyze the synthesis of indigo and derivatives for textile dyeing
Molecular Systems Design & Engineering ( IF 3.2 ) Pub Date : 2021-11-30 , DOI: 10.1039/d1me00123j
Lei Chen ₁ , Jia-Kun Xu ₂ , Lianzhi Li ₃ , Shu-Qin Gao ₄ , Ge-Bo Wen ₄ , Ying-Wu Lin _{1,

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Affiliation

The old dye indigo is still demanded worldwide, whereas chemical synthesis of indigo may cause environmental problems. Alternatively, the biosynthesis of indigo is an eco-friendly approach. In this study, by protein design and engineering, we showed that human neuroglobin (Ngb) can be converted into an enzyme capable of efficient synthesis of indigo from indole. Three mutations were rationally designed to stabilize the protein (A15C), enhance H₂O₂ activation (H64D), and facilitate electron transfer (F49Y), respectively. Kinetic studies and product analysis revealed that the triple mutant A15C/H64D/F49Y Ngb exhibits an activity much higher than those of the engineered myoglobin mutants, without the need of expensive cofactors required for cytochromes P450. This enzyme can also efficiently catalyze the coupling oxidation of indole derivatives containing –Cl/–Br/–NO₂ groups, producing indigo derivative dyes, with a yield up to ∼90% and chemoselectivity up to ∼97%, respectively. Moreover, these synthesized dyes were successfully applied to dye cotton textiles with uniform colors such as blue, red, yellow, purple, and even green. Thus, we expect that the engineered enzyme of Ngb will have practical applications in the textile dyeing industry.

中文翻译：

神经红蛋白的设计和工程催化合成用于纺织染色的靛蓝及其衍生物

世界范围内仍然需要旧染料靛蓝，而靛蓝的化学合成可能会导致环境问题。或者，靛蓝的生物合成是一种环保方法。在这项研究中，通过蛋白质设计和工程，我们表明人类神经红蛋白 (Ngb) 可以转化为能够从吲哚有效合成靛蓝的酶。合理设计三个突变以稳定蛋白质（A15C），增强H ₂ O ₂分别激活 (H64D) 和促进电子转移 (F49Y)。动力学研究和产物分析表明，三重突变体 A15C/H64D/F49Y Ngb 的活性远高于工程化肌红蛋白突变体，无需细胞色素 P450 所需的昂贵辅因子。该酶还可以有效催化含有–Cl/–Br/–NO ₂基团的吲哚衍生物的偶联氧化，产生靛蓝衍生物染料，产率分别高达~90% 和化学选择性高达~97%。此外，这些合成的染料成功地应用于棉纺织品染色，颜色均匀，如蓝色、红色、黄色、紫色，甚至绿色。因此，我们预计 Ngb 工程酶将在纺织印染行业有实际应用。

更新日期：2021-12-14

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