To develop interfacial nanobubbles (INBs) into application fields, it is of great significance to understand the properties of INBs and their effect on the interaction of inter-particles. In this paper, the morphology of INBs on different hydrophobic surfaces was researched by total internal reflection fluorescence microscopy (TIRF) and atomic force microscope (AFM). A hydrophobic AFM colloid cantilever was used to measure the interaction of inter-particles in the absence and presence of INBs. The results confirm that the formation of INBs is closely related to the hydrophobicity of substrates, more large INBs form on the strong hydrophobic surface (SS). Furthermore, the magnitude and range of the hydrophobic interaction force are positive related to the dimension of INBs. Besides, multiple approaches of hydrophobic colloid to SS, the hydrophobic interaction is further enhanced due to the merging and cavitation of INBs. Therefore, the results presented here provide the relationship between INBs formation and surface hydrophobicity, as well as the effect of INBs on particles interaction. The formation of INBs should be responsible for the hydrophobic interaction of inter-particles, which is a basic insight into the hydrophobic interaction between particles.

为了将界面纳米气泡（INBs）发展到应用领域，了解INBs的性质及其对颗粒间相互作用的影响具有重要意义。本文采用全内反射荧光显微镜（TIRF）和原子力显微镜（AFM）研究了INBs在不同疏水表面的形貌。疏水性 AFM 胶体悬臂用于测量在不存在和存在 INB 时粒子间的相互作用。结果证实，INBs的形成与底物的疏水性密切相关，在强疏水表面（SS）上形成了更多的大INBs。此外，疏水相互作用力的大小和范围与INBs的尺寸呈正相关。此外，疏水胶体对SS的多种途径，由于INB的合并和空化，疏水相互作用进一步增强。因此，此处提供的结果提供了 INB 形成与表面疏水性之间的关系，以及 INB 对粒子相互作用的影响。INB的形成应该是颗粒间疏水相互作用的原因，这是对颗粒之间疏水相互作用的基本认识。