ABSTRACT

Resveratrol is synthesized by the catalysis of resveratrol synthases (RS) in a limited number of higher plants. Resveratrol shows potential health-promoting properties, including as an antioxidant and in preventing cardiovascular diseases. Recently, resveratrol-enriched rice has been produced as a novel source of resveratrol. This study aimed to investigate the major agronomic characteristics of resveratrol-enriched rice, Iksan526 (I526) and compared them with those of a nontransgenic and commercial rice variety, Dongjin (DJ). Transgene (RS) integration was confirmed using Southern blot analysis, and homologous recombination was achieved after digestion with the SacI restriction enzyme. The phenotypic traits of I526 grown in Iksan were similar to those grown in Milyang but not similar to those grown in Suwon. In Suwon, I526 had slightly earlier heading dates [i.e., number of days from sowing to heading) and shorter culm lengths. When I526 was treated with 0.4% Basta in the seedling stage, no significant difference was observed among all the agronomic traits compared with nontreated I526; particularly, the culm length, panicle length, number of panicles per hill, 1,000 grain weight of brown rice, and brown rice yield of the Basta-treated rice were similar to those of the nontreated I526, regardless of their cultivation region. The resveratrol content of I526 grown in Suwon and Milyang was increased by 18% and 37%, respectively, than that of I526 grown in the Iksan area. Therefore, DJ and I526 are not significantly different in terms of major agronomic traits depending on variety/year and variety/cultivation region. The results indicated that I526 has the potential to become a commercialized variety in the near future.

摘要

白藜芦醇是在有限数量的高等植物中通过白藜芦醇合酶 (RS) 的催化合成的。白藜芦醇显示出潜在的促进健康的特性，包括作为抗氧化剂和预防心血管疾病。最近，已生产出富含白藜芦醇的大米作为白藜芦醇的新来源。本研究旨在调查富含白藜芦醇的水稻 Iksan526 (I526) 的主要农艺学特征，并将其与非转基因和商品化水稻品种 Dongjin (DJ) 的农艺学特征进行比较。使用 Southern 印迹分析确认转基因 (RS) 整合，并在用 SacI 限制酶消化后实现同源重组。益山种植的I526的表型性状与密阳种植的相似，但与水原种植的不相似。在水原，I526 抽穗日期稍早（即从播种到抽穗的天数）和较短的秆长度。I526在苗期用0.4%Basta处理时，与未处理的I526相比，所有农艺性状均无显着差异；特别是，无论种植区域如何，Basta处理水稻的秆长、穗长、穗数、糙米千粒重和糙米产量与未处理的I526相似。与益山地区种植的I526相比，水原和密阳种植的I526的白藜芦醇含量分别增加了18%和37%。因此，DJ 和 I526 在主要农艺性状方面没有显着差异，具体取决于品种/年份和品种/栽培区域。