Lactoferrin Alleviates Acute Alcoholic Liver Injury by Improving Redox-Stress Response Capacity in Female C57BL/6J Mice,Journal of Agricultural and Food Chemistry

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Lactoferrin Alleviates Acute Alcoholic Liver Injury by Improving Redox-Stress Response Capacity in Female C57BL/6J Mice
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-12-07 , DOI: 10.1021/acs.jafc.1c06813
Deming Li ₁ , Zhiqiang Hu ₁ , Qian He ₁ , Yaxin Guo ₁ , Yu Chong ₂ , Jiaying Xu ₂ , Liqiang Qin ₁

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Lactoferrin (Lf) can attenuate alcoholic liver injury (ALI) in male mice; however, the effects of Lf on acute ALI in female mice are still unknown. Female C57BL/6J mice were randomly divided into four groups and fed with different diets for 4 weeks: an AIN-93G diet for control (CON) and ethanol (EtOH) groups; an AIN-93G diet with 0.4 and 4% casein replaced by Lf for low-dose Lf (LLf) and high-dose Lf (HLf) groups. Acute ALI was induced by intragastric administration of ethanol (4.8 g/kgbw) every 12 h continuously for three times. HLf had obvious alleviating effects on acute ALI. Lf pretreatment did not affect hepatic alcohol metabolism key enzymes. Meanwhile, the ethanol-induced hepatic reactive oxygen species level increase was not ameliorated by Lf. Metabolomics and bioinformatics analysis results suggested an important role of redox-stress response capacity (RRC). Western blots showed HLf-promoted AKT and AMP-activated protein kinase activations and upregulated Nrf2 and LC3-II expressions, which was associated with RRC improvement. In summary, HLf could prevent acute ALI in female mice, and RRC likely played an important role.

中文翻译：

乳铁蛋白通过提高雌性 C57BL/6J 小鼠的氧化还原应激反应能力减轻急性酒精性肝损伤

乳铁蛋白 (Lf) 可以减轻雄性小鼠的酒精性肝损伤 (ALI)；然而，Lf 对雌性小鼠急性 ALI 的影响仍然未知。雌性 C57BL/6J 小鼠被随机分为四组，并用不同的饮食喂养 4 周：AIN-93G 饮食用于对照组 (CON) 和乙醇 (EtOH) 组；对于低剂量 Lf (LLf) 和高剂量 Lf (HLf) 组，AIN-93G 饮食用 0.4% 和 4% 酪蛋白替换为 Lf。急性 ALI 是通过连续 3 次每 12 小时灌胃乙醇（4.8 g/kgbw）诱导的。HLf对急性ALI有明显的缓解作用。Lf预处理不影响肝脏酒精代谢关键酶。同时，乙醇诱导的肝脏活性氧水平增加并没有被 Lf 改善。代谢组学和生物信息学分析结果表明氧化还原应激反应能力 (RRC) 的重要作用。蛋白质印迹显示 HLf 促进 AKT 和 AMP 激活的蛋白激酶激活，并上调 Nrf2 和 LC3-II 表达，这与 RRC 改善有关。总之，HLf 可以预防雌性小鼠的急性 ALI，而 RRC 可能发挥了重要作用。

更新日期：2021-12-15

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