Mycoplasma spp. are important pathogens in poultry and cause high economic losses for poultry industry worldwide. In other bird species (e.g. white storks, birds of prey, and several waterfowl species), Mycoplasma spp. are regularly found in healthy individuals, hence, considered apathogenic or part of the microbiota of the upper respiratory tract. However, as Mycoplasma spp. are absent in healthy individuals of some wild bird species, they might play a role as respiratory pathogen in these bird species, e.g. Mycoplasma gallisepticum in house finches. The knowledge on the occurrence of Mycoplasma spp. in wild birds is limited. To evaluate the relevance of Mycoplasma spp. in free-ranging nightingales and tits, 172 wild caught birds were screened for the presence of mycoplasmas. The birds were sampled via choanal swabs and examined via molecular methods (n = 172) and, when possible, via culture (n = 142). The Mycoplasma sp. was determined by sequencing the 16S rRNA gene and 16S-23S Intergenic Transcribed Spacer Region. All birds were tested negative for mycoplasmas via PCR and/or mycoplasmal culture. Hence, free-ranging nightingales and tits do not show any mycoplasma in their microbial flora of the respiratory tract. Therefore, these songbird species may suffer from clinical mycoplasmosis when being infected. We hypothesize that birds relying on their vocal ability for reproduction have excluded mycoplasmas from their respiratory flora compared to other bird species.

支原体属。是家禽中的重要病原体，给全世界的家禽业造成巨大的经济损失。在其他鸟类（例如白鹳、猛禽和几种水禽）中，支原体属。经常在健康个体中发现，因此被认为是上呼吸道微生物群的一部分。然而，作为支原体。在一些野生鸟类的健康个体中不存在，它们可能在这些鸟类中作为呼吸道病原体发挥作用，例如家雀中的鸡毒支原体。关于支原体发生的知识。在野生鸟类中是有限的。评估支原体的相关性属 在自由放养的夜莺和山雀中，对 172 只野生捕获的鸟类进行了支原体筛查。通过后鼻拭子对鸟类进行采样，并通过分子方法 ( n  = 172)进行检查，并在可能的情况下通过培养 ( n  = 142) 进行检查。该支原体sp. 通过对 16S rRNA 基因和 16S-23S 基因间转录间隔区进行测序来确定。通过 PCR 和/或支原体培养，所有鸡的支原体检测均呈阴性。因此，自由放养的夜莺和山雀的呼吸道微生物群中没有任何支原体。因此，这些鸣禽物种在被感染时可能会患上临床支原体病。我们假设与其他鸟类相比，依靠声音繁殖能力的鸟类已将支原体排除在呼吸道菌群之外。