Deep eutectic solvents – a new additive in the encapsulation of lipase B from Candida antarctica: biocatalytic applications,Reaction Chemistry & Engineering

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Deep eutectic solvents – a new additive in the encapsulation of lipase B from Candida antarctica: biocatalytic applications
Reaction Chemistry & Engineering ( IF 3.4 ) Pub Date : 2021-11-23 , DOI: 10.1039/d1re00469g
Adrian Ioan Dudu ₁ , Laszlo Csaba Bencze ₁ , Csaba Paizs ₁ , Monica Ioana Toşa ₁

Affiliation

Enzyme encapsulation in a sol–gel matrix can be improved using some additives acting on enzyme activity and/or stability. Ionic liquids, polyhydroxy compounds, sugars, etc. have been previously reported as additives. This study focuses on the possibility to use deep eutectic solvents as additives in the immobilization of lipase B from Candida antarctica (CaL-B) through sol–gel encapsulation. The transesterification of 1-phenylethanol was chosen as a model reaction. Process optimization was successfully performed regarding the most important reaction parameters (an alcohol/lipase weight ratio of 20 : 1, an alcohol/acyl donor molar ratio of 1 : 2, n-hexane as a solvent, 30 °C reaction temperature, vinyl acetate as an acyl donor and a substrate concentration of 100 mM). Under the optimal conditions, the reaction mediated by the most active biocatalyst (SG-3, containing a deep eutectic solvent formed from choline chloride and fructose, as a 30% aqueous solution) reached ∼48% after only four hours, maintaining almost 85% of its initial activity even after eight reaction cycles.

中文翻译：

深共熔溶剂——一种用于包裹南极念珠菌脂肪酶 B 的新添加剂：生物催化应用

使用一些影响酶活性和/或稳定性的添加剂可以改善溶胶-凝胶基质中的酶包封。离子液体、多羟基化合物、糖等先前已被报道为添加剂。本研究的重点是在通过溶胶-凝胶封装固定来自南极念珠菌(CaL-B)的脂肪酶 B 中使用深共熔溶剂作为添加剂的可能性。选择 1-苯基乙醇的酯交换作为模型反应。关于最重要的反应参数（醇/脂肪酶重量比为 20:1，醇/酰基供体摩尔比为 1:2，n-己烷作为溶剂，反应温度为 30 °C，乙酸乙烯酯作为酰基供体，底物浓度为 100 mM）。在最佳条件下，由活性最强的生物催化剂（SG-3，含有氯化胆碱和果糖形成的深共熔溶剂，30% 水溶液）介导的反应仅 4 小时后达到约 48%，保持近 85%即使在八个反应循环后其初始活性。

更新日期：2021-12-07

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