ABSTRACT

As a common malignant tumor, colorectal cancer (CRC) has a high incidence. Recent investigations have suggested that although great improvement has been achieved in the survival rate of early-stage CRC patients, the overall survival rate remains low. Mounting reports have proved that lncRNAs take part in the development of various cancers and possess the regulatory functions in cancers. For example, ASB16 antisense RNA 1 (ASB16-AS1) is a poorly researched novel lncRNA whose specific functions in CRC are still unknown. In our research, we discovered that ASB16-AS1 was with high expression in CRC cells. In addition, ASB16-AS1 silencing restrained the proliferation, migration, invasion, and stemness while accelerating cell apoptosis of CRC cells. Mechanism experiments were applied to explore the regulatory mechanism of ASB16-AS1. It turned out that miR-185-5p could interact with ASB16-AS1 and inhibited the progression of CRC cells. TEAD1 (TEA domain transcription factor1) - a major effector of the Hippo signaling was proved to serve as the target of miR-185-5p and promote CRC development. In short, ASB16-AS1 drove the progression of CRC through the regulation of miR-185-5p/TEAD1 axis.

摘要

结直肠癌（CRC）作为一种常见的恶性肿瘤，发病率很高。最近的研究表明，尽管早期 CRC 患者的生存率已经取得了很大的改善，但总体生存率仍然很低。越来越多的报道证明，lncRNA参与了多种癌症的发生发展，并在癌症中具有调控功能。例如，ASB16 反义 RNA 1 (ASB16-AS1) 是一种研究较少的新型 lncRNA，其在 CRC 中的具体功能仍不清楚。在我们的研究中，我们发现 ASB16-AS1 在 CRC 细胞中高表达。此外，ASB16-AS1沉默抑制了CRC细胞的增殖、迁移、侵袭和干性，同时加速了CRC细胞的细胞凋亡。采用机制实验探索ASB16-AS1的调控机制。事实证明，miR-185-5p 可以与 ASB16-AS1 相互作用并抑制 CRC 细胞的进展。TEAD1（TEA 结构域转录因子 1） - Hippo 信号传导的主要效应物被证明可作为 miR-185-5p 的靶标并促进 CRC 发展。简而言之，ASB16-AS1 通过调节 miR-185-5p/TEAD1 轴来驱动 CRC 的进展。