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Butanol production by Clostridium acetobutylicum ATCC 824 using electro-fermentation in culture medium supplemented with butyrate and neutral red
Journal of Chemical Technology and Biotechnology ( IF 2.8 ) Pub Date : 2021-12-06 , DOI: 10.1002/jctb.7007
Karlo Guerrero 1 , Roberto Gallardo 2 , Ernesto González 1 , Fabián Veliz 3 , Raúl Conejeros 1 , Juan Carlos Gentina 1 , Germán Aroca 1
Affiliation  

Butanol is a versatile chemical that can be used as fuel, solvent or intermediate in chemical synthesis. Several strategies have been used to increase the availability of reducing power in Clostridium sp. with the aim of maximizing the production of butanol, the use of electron carriers being one of the most effective. Moreover, electrons from an external power source can be transferred to cells through electron carriers such neutral red, or directly reducing NAD+. From a previous metabolic analysis, we hypothesized that adding butyrate to the culture medium and using an external electron source as a co-reducing agent, along with glucose-derived reducing power (electro-fermentation), would result in an increase in the specific butanol productivity.

中文翻译:

丙酮丁醇梭菌 ATCC 824 在添加丁酸盐和中性红的培养基中电发酵生产丁醇

丁醇是一种用途广泛的化学品,可用作化学合成中的燃料、溶剂或中间体。已经使用了几种策略来增加梭状芽孢杆菌中还原能力的可用性。为了最大限度地生产丁醇,使用电子载体是最有效的方法之一。此外,来自外部电源的电子可以通过电子载体(如中性红)或直接还原 NAD +转移到细胞中。根据之前的代谢分析,我们假设在培养基中添加丁酸盐并使用外部电子源作为共还原剂,以及葡萄糖衍生的还原能力(电发酵),会导致特定丁醇的增加生产率。
更新日期:2021-12-06
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