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Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-12-06 , DOI: 10.1021/acs.jafc.1c07050 Mengqi Li 1 , Renzhi Lv 1 , Xiaomeng Xu 1 , Qi Ge 1 , Songyi Lin 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-12-06 , DOI: 10.1021/acs.jafc.1c07050 Mengqi Li 1 , Renzhi Lv 1 , Xiaomeng Xu 1 , Qi Ge 1 , Songyi Lin 1
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Acute gastric injury caused by ethanol is a frequent disorder of the gastrointestinal tract. In this study, we investigated the potential gastroprotective effects of Tricholoma matsutake-derived peptides against ethanol-triggered acute gastric injury and the associated mechanisms. Peptides SDLKHFPF and SDIKHFPF significantly attenuated the ethanol-induced decrease in GES-1 cell survival (82.39 ± 1.93 and 80.10 ± 1.08% vs 56.58 ± 1.86%), inhibited GES-1 cell apoptosis, and alleviated the ethanol-induced gastric mucosal injury (64.76 ± 3.98 and 49.29 ± 3.25%), ulcer index (3.33 ± 0.47 and 4.67 ± 0.47 vs 6.67 ± 0.47), and histopathological changes in mice. Peptide treatment inhibited the phosphorylation and nuclear translocation of nuclear factor kappa B (NF-κB), the secretion of tumor necrosis factor-α, interleukin-6, and endothelin-1. In addition, T. matsutake peptide pretreatment increased growth factor secretion, upregulated B-cell lymphoma-2, downregulated Bcl-2-associated X (Bax), and cleaved cysteinyl aspartate specific proteinase 3, thereby promoting gastric cell survival. These findings strongly suggest that T. matsutake peptides attenuate ethanol-induced inflammatory responses and apoptosis by suppressing NF-κB signaling activation, thereby enhancing gastric epithelial barrier functions.
中文翻译：
Tricholoma matsutake 衍生肽对乙醇诱导的急性胃损伤显示出胃保护作用
乙醇引起的急性胃损伤是一种常见的胃肠道疾病。在这项研究中，我们研究了松茸的潜在胃保护作用抗乙醇引发的急性胃损伤的衍生肽及其相关机制。肽 SDLKHFPF 和 SDIKHFPF 显着减弱乙醇诱导的 GES-1 细胞存活率下降（82.39 ± 1.93 和 80.10 ± 1.08% vs 56.58 ± 1.86%），抑制 GES-1 细胞凋亡，并减轻乙醇诱导的胃粘膜损伤（ 64.76 ± 3.98 和 49.29 ± 3.25%）、溃疡指数（3.33 ± 0.47 和 4.67 ± 0.47 vs 6.67 ± 0.47）和小鼠的组织病理学变化。肽处理抑制了核因子κB (NF-κB) 的磷酸化和核转位、肿瘤坏死因子-α、白细胞介素-6 和内皮素-1 的分泌。此外，T. matsutake肽预处理增加生长因子分泌，上调 B 细胞淋巴瘤-2，下调 Bcl-2 相关 X (Bax)，并裂解半胱氨酰天冬氨酸特异性蛋白酶 3，从而促进胃细胞存活。这些发现有力地表明，松茸肽通过抑制 NF-κB 信号激活来减弱乙醇诱导的炎症反应和细胞凋亡，从而增强胃上皮屏障功能。
更新日期：2021-12-15
中文翻译：
Tricholoma matsutake 衍生肽对乙醇诱导的急性胃损伤显示出胃保护作用
乙醇引起的急性胃损伤是一种常见的胃肠道疾病。在这项研究中，我们研究了松茸的潜在胃保护作用抗乙醇引发的急性胃损伤的衍生肽及其相关机制。肽 SDLKHFPF 和 SDIKHFPF 显着减弱乙醇诱导的 GES-1 细胞存活率下降（82.39 ± 1.93 和 80.10 ± 1.08% vs 56.58 ± 1.86%），抑制 GES-1 细胞凋亡，并减轻乙醇诱导的胃粘膜损伤（ 64.76 ± 3.98 和 49.29 ± 3.25%）、溃疡指数（3.33 ± 0.47 和 4.67 ± 0.47 vs 6.67 ± 0.47）和小鼠的组织病理学变化。肽处理抑制了核因子κB (NF-κB) 的磷酸化和核转位、肿瘤坏死因子-α、白细胞介素-6 和内皮素-1 的分泌。此外，T. matsutake肽预处理增加生长因子分泌，上调 B 细胞淋巴瘤-2，下调 Bcl-2 相关 X (Bax)，并裂解半胱氨酰天冬氨酸特异性蛋白酶 3，从而促进胃细胞存活。这些发现有力地表明，松茸肽通过抑制 NF-κB 信号激活来减弱乙醇诱导的炎症反应和细胞凋亡，从而增强胃上皮屏障功能。
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