Brown root rot (BRR) caused by Phellinus noxius is a destructive tree disease in tropical and subtropical areas. To understand how BRR affects the composition of the plant rhizoplane-enriched microbiota, the microbiomes within five root-associated compartments (i.e., bulk soil, old/young root rhizosphere soil, old/young root tissue) of Ficus trees naturally infected by P. noxius were investigated. The level of P. noxius infection was determined by quantitative PCR. Illumina sequencing of the internal transcribed spacer and 16S rRNA revealed that P. noxius infection caused a significant reduction in fungal diversity in the bulk soil, the old root rhizosphere soil, and the old root tissue. Interestingly, Cosmospora was the only fungal genus positively correlated with P. noxius. The abundance and composition of dominant bacterial taxa such as Actinomadura, Bacillus, Rhodoplanes, and Streptomyces differed between BRR-diseased and healthy samples. Furthermore, 838 isolates belonging to 26 fungal and 35 bacterial genera were isolated and tested for interactions with P. noxius. Antagonistic activities were observed for isolates of Bacillus, Pseudomonas, Aspergillus, Penicillium, and Trichoderma. Cellophane overlay and cellulose/lignin utilization assays suggested that Cosmospora could tolerate the secretions of P. noxius and that the degradation of lignin by P. noxius may create suitable conditions for Cosmorpora growth.

中文翻译：

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褐根腐真菌桑黄影响榕树不同根相关生态位中的微生物群落

由桑黄引起的褐根腐病（BRR）是热带和亚热带地区的一种破坏性树木病害。为了了解 BRR 如何影响植物富含根际的微生物群的组成，研究了自然感染P. noxius进行了调查。通过定量 PCR 确定P. noxius感染的水平。内部转录间隔区和 16S rRNA 的 Illumina 测序显示，P. noxius感染导致大块土壤、老根根际土壤和老根组织中的真菌多样性显着降低。有趣的是，Cosmospora是唯一与P. noxius呈正相关的真菌属。BRR 患病样本和健康样本之间主要细菌类群（如Actinomadura、Bacillus、Rhodoplanes和Streptomyces ）的丰度和组成不同。此外，分离出属于 26 个真菌属和 35 个细菌属的 838 个分离物，并测试了与P. noxius的相互作用。对芽孢杆菌属、假单胞菌属、曲霉属、青霉属和木霉属的分离株观察到拮抗活性. 玻璃纸覆盖层和纤维素/木质素利用测定表明，大孢菌可以耐受 P. noxius 的分泌物，并且P. noxius对木质素的降解可能为Cosmorpora的生长创造合适的条件。