Raphanobrassica (RrRrCrCr, 2n = 4x = 36), which is generated by distant hybridization between the maternal parent Raphanus sativus (RsRs, 2n = 2x = 18) and the paternal parent Brassica oleracea (C°C°, 2n = 2x = 18), displays intermediate silique phenotypes compared to diploid progenitors. However, the hybrid shares much more similarities in silique phenotypes with those of B. oleracea than those of R. sativus. Strikingly, the silique of Raphanobrassica is obviously split into two parts. To investigate the gene expression patterns behind these phenomena, transcriptome analysis was performed on the upper, middle, and lower sections of pods (RCsiu, RCsim, and RCsil), seeds in the upper and lower sections of siliques (RCseu and RCsel) from Raphanobrassica, whole pods (Rsi and Csi) and all seeds in the siliques (Rse and Cse) from R. sativus and B. oleracea. Transcriptome shock was observed in all five aforementioned tissues of Raphanobrassica. Genome-wide unbalanced biased expression and expression level dominance were also discovered, and both of them were toward B. oleracea in Raphanobrassica, which is consistent with the observed phenotypes. The present results reveal the global gene expression patterns of different sections of siliques of Raphanobrassica, pods, and seeds of B. oleracea and R. sativus, unraveling the tight correlation between global gene expression patterns and phenotypes of the hybrid and its parents.

中文翻译：

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人工合成的萝卜属间杂种中甘蓝的全基因组不平衡表达偏向和表达水平优势


Raphanobrassica (RrRrCrCr, 2n = 4x = 36)，由母本 Raphanus sativus (RsRs, 2n = 2x = 18) 和父本 Brassica oleracea (C°C°, 2n = 2x = 18) 之间的远缘杂交产生，与二倍体祖细胞相比，显示出中间长角果表型。然而，该杂交种在长角果表型方面与甘蓝的相似性远高于与红花的相似性。引人注目的是，萝卜的长角果明显分成两部分。为了研究这些现象背后的基因表达模式，对萝卜荚的上、中、下段（RCsiu、RCsim 和 RCsil）、长角果上段和下段的种子（RCseu 和 RCsel）进行了转录组分析。 、整个豆荚（Rsi 和 Csi）以及来自 R. sativus 和 B. oleracea 的长角果中的所有种子（Rse 和 Cse）。在上述所有五个萝卜组织中均观察到转录组休克。还发现了全基因组不平衡的偏向表达和表达水平优势，并且两者都朝向萝卜中的甘蓝，这与观察到的表型一致。目前的结果揭示了萝卜、甘蓝和R. sativus的角果、豆荚和种子的不同部分的整体基因表达模式，揭示了整体基因表达模式与杂种及其亲本表型之间的紧密相关性。