Water dropwort (Liyang Baiqin, Oenanthe javanica (BI.) DC.) is an aquatic perennial plant from the Apiaceae family with abundant protein, dietary fiber, vitamins, and minerals. It usually grows in wet soils and can even grow in water. Here, whole-genome sequencing of O. javanica via HiSeq 2000 sequencing technology was reported for the first time. The genome size was 1.28 Gb, including 42,270 genes, of which 93.92% could be functionally annotated. An online database of the whole-genome sequences of water dropwort, Water dropwortDB, was established to share the results and facilitate further research on O. javanica (database homepage: http://apiaceae.njau.edu.cn/waterdropwortdb). Water dropwortDB offers whole-genome and transcriptome sequences and a Basic Local Alignment Search Tool. Comparative analysis with other species showed that the evolutionary relationship between O. javanica and Daucus carota was the closest. Twenty-five gene families of O. javanica were found to be expanded, and some genetic factors (such as genes and miRNAs) related to phenotypic and anatomic differentiation in O. javanica under different water conditions were further investigated. Two miRNA and target gene pairs (miR408 and Oja15472, miR171 and Oja47040) were remarkably regulated by water stress. The obtained reference genome of O. javanica provides important information for future work, thus making in-depth genetic breeding and gene editing possible. The present study also provides a foundation for the understanding of the O. javanica response to water stress, including morphological, anatomical, and genetic differentiation.

中文翻译：

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水滴蒿 (Oenanthe javanica) 响应水分胁迫的基因组、转录组、microRNA 和降解组分析

水芹（溧阳白芹、爪哇月见草(BI.) DC.) 是伞形科多年生水生植物，富含蛋白质、膳食纤维、维生素和矿物质。它通常生长在潮湿的土壤中，甚至可以在水中生长。在这里，全基因组测序爪哇首次报道了通过HiSeq 2000测序技术。基因组大小为 1.28 Gb，包括 42,270 个基因，其中 93.92% 可以进行功能注释。建立了水芹全基因组序列在线数据库Water dropwortDB，以共享结果并促进进一步研究爪哇（数据库主页：http://apiaceae.njau.edu.cn/waterdropwortdb）。Water dropwortDB 提供全基因组和转录组序列以及基本的局部比对搜索工具。与其他物种的比较分析表明，两者之间的进化关系爪哇和胡萝卜是最接近的。二十五个基因家族爪哇发现与表型和解剖学分化相关的一些遗传因素（如基因和miRNA）爪哇进一步研究了在不同的水质条件下。两个 miRNA 和靶基因对（miR408 和奥哈15472, miR171 和Oja47040) 受到水分胁迫的显着调节。获得的参考基因组爪哇为未来的工作提供重要信息，从而使深入的遗传育种和基因编辑成为可能。本研究也为理解本研究提供了基础爪哇对水分胁迫的反应，包括形态学、解剖学和遗传分化。