Nowadays, microplastics (MPs) and adsorbed pollutants are considered a global thread to marine ecosystems. This study describes the effects of pollutants and MPs ingestion on fish brains through the assessment of oxidative stress biomarkers and monoaminergic neurotransmitters using gilthead seabream (Sparus aurata) as fish model. Juveniles were experimentally exposed to three different dietary treatments for 90 days: Control treatment (C) consisted of standard feed; Virgin treatment (V) contained feed enriched with 10% of MPs; and Exposed treatment (E) consisted of feed with 10% of MPs that were exposed to seawater in an anthropogenically impacted area for 2 months in order to enrich the plastic with the pollutants within the water column. Sampling was made at the start of the experiment (T0), at the end of the dietary treatments (T90) and after a posterior detoxification period of 30 days (T120). Results evidenced that a MPs and pollutants enriched diet increases the activity of some of the oxidative stress biomarkers (e.g. CAT and GST), and it was shown for the first time alterations on dopaminergic and serotonergic system activity on seabream brains, indicating potential neurofunctional effects associated to MPs and pollutants ingestion. In addition, results showed a tendency to recover enzymatic and brain monoaminergic neurotransmitter levels after a 30-day detoxification period. In conclusion, MPs and pollutants exposure for 90 days induced oxidative stress and changes on monoaminergic activity in the brain of S. aurata.

如今，微塑料 (MPs) 和吸附的污染物被认为是海洋生态系统的全球主线。本研究通过使用金头鲷 ( Sparus aurata ) 评估氧化应激生物标志物和单胺能神经递质，描述了污染物和 MPs 摄入对鱼脑的影响。) 作为鱼模型。幼鱼实验性地暴露于三种不同的日粮处理 90 天： 对照处理 (C) 由标准饲料组成；原始处理 (V) 包含富含 10% MPs 的饲料；暴露处理 (E) 包括在人为影响区域暴露于海水 2 个月的含有 10% MPs 的饲料，以使塑料富含水体中的污染物。在实验开始时 (T0)、饮食处理结束时 (T90) 和 30 天的后排毒期 (T120) 进行采样。结果表明，富含 MPs 和污染物的饮食增加了一些氧化应激生物标志物（例如 CAT 和 GST）的活性，并且首次显示了鲷脑多巴胺能和血清素能系统活性的改变，表明与 MPs 和污染物摄入相关的潜在神经功能影响。此外，结果显示在 30 天的解毒期后，酶和脑单胺能神经递质水平有恢复的趋势。总之，MPs 和污染物暴露 90 天会诱发氧化应激和大脑中单胺能活性的变化。S. aurata。