Overexpression or activation of Yes-associated protein (YAP) is common in cancer cells. Thus, targeting YAP may be a strategy for cancer therapy. Licochalcone A (LicA) is a primary active compound of licorice root and is known to have medicinal effects, such as antioxidant, antibacterial, antiviral, and anticancer effects. However, the anticancer pharmacological mechanism of LicA has not been investigated in cholangiocarcinoma. In this study, we investigated the antiproliferative effect of LicA and the underlying molecular mechanism in HCCC-9810 and RBE human cholangiocarcinoma cells. Our experiments indicated that LicA suppressed the growth of cholangiocarcinoma cells through inactivation of the Hippo pathway. Pescadillo ribosomal biogenesis factor 1 (PES1) was notably upregulated and related to carcinogenesis. We also found that LicA suppressed the expression and nuclear localization of PES1, which was associated with the inhibition of YAP expression and transcriptional activity.

中文翻译：

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甘草查耳酮 A 通过 PES1 下调 Hippo 通路在胆管癌细胞中抑制细胞生长

Yes 相关蛋白 (YAP) 的过度表达或激活在癌细胞中很常见。因此，靶向 YAP 可能是癌症治疗的一种策略。甘草查耳酮 A (LicA) 是甘草根的主要活性化合物，已知具有抗氧化、抗菌、抗病毒和抗癌等药用作用。然而，尚未在胆管癌中研究 LicA 的抗癌药理机制。在这项研究中，我们研究了 LicA 的抗增殖作用及其在 HCCC-9810 和 RBE 人胆管癌细胞中的潜在分子机制。我们的实验表明，LicA 通过使 Hippo 通路失活来抑制胆管癌细胞的生长。Pescadillo 核糖体生物发生因子 1 (PES1) 显着上调并与致癌作用有关。