Extracellular vesicles (EVs) released by tumor cells play important roles on the remodeling of the tumor–stromal environment and on promoting tumor metastasis. Our earlier studies revealed that miR-122-5p, a type of small non-coding RNA, was dysregulated in non-small cell lung cancer (NSCLC) cell-derived EVs. In this study, we found that miR-122-5p was selectively sorted and secreted into lung cancer EVs through binding to RNA-binding protein hnRNPA2B1. In addition, we found that hnRNPA2B1 interacted with miR-122-5p through the EXO-motif. The delivering of lung cancer EVs-miR-122-5p promoted the migration of liver cells, which may play roles in establishing a pre-metastatic micro-environment and hepatic metastasis of lung cancer. Importantly, our findings revealed the molecular mechanism that RNA-binding protein controls the selective sorting of tumor-derived EV miR-122-5p, which potentially promotes lung cancer progression.

中文翻译：

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hnRNPA2B1 介导的 miR-122-5p 细胞外囊泡分选可能促进肺癌进展

肿瘤细胞释放的细胞外囊泡（EVs）在重塑肿瘤-基质环境和促进肿瘤转移方面发挥着重要作用。我们早期的研究表明，miR-122-5p 是一种小型非编码 RNA，在非小细胞肺癌 (NSCLC) 细胞衍生的 EV 中失调。在这项研究中，我们发现 miR-122-5p 通过与 RNA 结合蛋白 hnRNPA2B1 结合而选择性地分选并分泌到肺癌 EVs 中。此外，我们发现 hnRNPA2B1 通过 EXO 基序与 miR-122-5p 相互作用。肺癌EVs-miR-122-5p的递送促进了肝细胞的迁移，这可能在肺癌转移前微环境的建立和肝转移中发挥作用。重要的，