Despite the active development of microchannel devices for cell manipulation, there are few reports on such devices for plants than those for animal cells, including human cells. This paper presents root-hair isolation and observation methods for Arabidopsis thaliana roots by using a microchannel device. Specifically, to facilitate observation, the height and width of the channel were optimized so that only one A. thaliana root hair could pass through. As A. thaliana is a model organism for plant research, in this study, it was used in a experiment with the expectation that its application will expand in the future. In typical in vitro experimental systems, a gel-like medium is used; however, as this is difficult to introduce into the micron-order narrow space of a microchannel device, a liquid medium was used in this study. We established a protocol unique to microchannel devices and succeeded in isolating root hairs at channel heights and widths of at least 10 μm and 10–14 μm, respectively. Root-hair elongation was confirmed in the microchannel, and the deceleration of root-hair elongation rate was confirmed quantitatively. In a future work, based on the findings of this study, we intend to analyze the behavior of individual root hairs and clarify the correlation with repellent substances by exploiting the characteristics of microchannel devices. This research can potentially serve as a basis for future applied research of agricultural chemicals.

尽管用于细胞操作的微通道设备的积极发展，但很少有关于植物的此类设备的报道，而不是动物细胞（包括人体细胞）的设备。本文介绍了使用微通道装置对拟南芥根进行根毛分离和观察的方法。具体来说，为了便于观察，优化了通道的高度和宽度，以便只有一根拟南芥根毛可以通过。由于拟南芥是植物研究的模式生物，因此本研究将其用于实验中，期望在未来扩大其应用范围。在典型的体外实验系统，使用凝胶状介质；然而，由于这很难引入微通道装置的微米级狭窄空间，因此本研究使用了液体介质。我们建立了微通道设备独有的协议，并成功地在通道高度和宽度至少为 10 μ m 和 10-14 μ处隔离根毛m，分别。在微通道中确认根毛伸长，定量确认根毛伸长率的减慢。在未来的工作中，基于本研究的结果，我们打算通过利用微通道设备的特性来分析单个根毛的行为并阐明与驱避物质的相关性。这项研究有可能成为未来农用化学品应用研究的基础。