For several years, orbitally shaken cylindrical bioreactors (OSRs) have become a new approach for propagation of mammalian cells cultivated under suspension. With new commercial cell culture media available today, allowing exceptionally high cell densities, the identification of suitable operating conditions presents a special challenge. Balancing hydrodynamic stress and other factors in reactors, this is particularly true for gas transfer rates providing sufficient oxygen (O₂) to cultures that can attain ten-fold greater biomasses than just a decade ago. This challenge is more profound the larger the working volume of the reactor becomes.

中文翻译：

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不同操作条件下中试规模的 OrbShake 生物反应器的流体动力学

几年来，轨道摇动圆柱形生物反应器 (OSR) 已成为繁殖悬浮培养的哺乳动物细胞的新方法。当今可使用新的商业细胞培养基，允许异常高的细胞密度，确定合适的操作条件提出了特殊的挑战。_{平衡反应器中的流体动力应力和其他因素，这对于为培养物提供足够氧气 (O 2} )的气体传输速率尤其如此，这些培养物可以获得比十年前多十倍的生物量。反应器的工作体积越大，这一挑战就越严重。