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Growth promotion of interspecific hybrid embryos between Cucumis anguria and Melon (C. melo) by fruit heating
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2021-11-25 , DOI: 10.1016/j.hpj.2021.11.013
Yuichi Matsumoto 1 , Mako Tsunamoto 1
Affiliation  

Wild species of the genus Cucumis, such as C. anguria, are resistant to various melon (C. melo) diseases such as Fusarium wilt race 1.2 and are anticipated as genetic resources of melon. However, in the interspecific crosses between C. anguria and melon, the abortion of the embryos was observed in the early growth stage and could not be regenerated even when cultured. Therefore, for embryo rescue, it is necessary to promote embryo growth during abortion occurrence. This study investigated the effects of fruit heating on embryo growth in interspecific hybrids of C. anguria and melon. In the heating device, the minimum night temperature around the fruit was maintained above 21.5 °C. The ovule grain area of C. anguria × MR-1 with fruit heating was significantly enlarged compared to that without fruit-heating. It was also significantly enlarged compared with the ovule grain area of C. anguria self-pollination. Although, in C. anguria × MR-1 without fruit-heating, no embryo was observed until 10 days after pollination (DAP), and pre-embryos were observed at 13 DAP. Conversely, in C. anguria × MR-1 with fruit-heating, pre-embryos were even observed at 10 DAP. These results suggest that the possibility of embryo or ovule culture regeneration has increased due to embryo growth promotion.



中文翻译:

果实加热促进黄瓜与甜瓜(C. melo)种间杂种胚的生长

黄瓜属的野生种,如C. anguria,对各种甜瓜 ( C. melo ) 病害如枯萎病菌 1.2 具有抗性,并有望成为甜瓜的遗传资源。然而,在C. anguria和甜瓜的种间杂交中,在早期生长阶段观察到胚胎流产,即使培养也不能再生。因此,对于胚胎抢救,有必要在流产发生时促进胚胎生长。本研究调查了果实加热对C. anguria和甜瓜种间杂种胚胎生长的影响。在加热装置中,水果周围的最低夜间温度保持在 21.5°C 以上。胚珠粒面积C. anguria  × MR-1 与没有果实加热的相比,果实加热显着增大。与C. anguria自花授粉的胚珠粒面积相比也显着增大。尽管在 没有果实加热的C. anguria × MR-1 中,直到授粉后 10 天(DAP)才观察到胚胎,并且在 13 DAP 观察到前胚胎。相反,在C. anguria  × MR-1 与果实加热,甚至在 10 DAP 观察到前胚。这些结果表明,由于胚胎生长促进,胚胎或胚珠培养物再生的可能性增加了。

更新日期:2021-11-25
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